Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,894,763	T→A	L190I (TTA→ATA)	ygcP →	putative anti‑terminator regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,894,763	0	T	A	100.0%	142.7 / NA	39	L190I (TTA→ATA)	ygcP	putative anti‑terminator regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/21);  total (18/21)

GCGCGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGC  >  NC_000913/2894688‑2894839                                                                            |                                                                             gcgcGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATATCGGGGTCTGGACGTTAGCGAAAAAAATACtt                                                                         >  2:535443/1‑81 (MQ=255) gcgcGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACtt                                                                         >  1:535455/1‑81 (MQ=255)  cgcgTAGTGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACttt                                                                        <  1:535403/81‑1 (MQ=255)  cgcgTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACttt                                                                        >  1:535444/1‑81 (MQ=255)   gcgTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTg                                                                       <  1:535404/81‑1 (MQ=255)   gcgTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATAATTTg                                                                       <  2:535405/81‑1 (MQ=255)         tGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGa                                                                 <  2:535406/81‑1 (MQ=255)           cGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATa                                                               <  1:535407/81‑1 (MQ=255)                aaCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCg                                                           >  2:535441/1‑80 (MQ=255)                     gggTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAAcc                                                      >  2:535442/1‑80 (MQ=255)                        tgtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAAtt                                                  <  1:535408/81‑1 (MQ=255)                          tCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGa                                                <  1:535409/81‑1 (MQ=255)                               gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTg                                           <  1:535412/81‑1 (MQ=255)                               gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTg                                           <  2:535410/81‑1 (MQ=255)                               gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTg                                           <  2:535411/81‑1 (MQ=255)                                    ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGttt                                       >  2:535447/1‑80 (MQ=255)                                     tCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTg                                      <  2:535414/80‑1 (MQ=255)                                     tCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGa                                     >  1:535448/1‑81 (MQ=255)                                     tCCACCACGAATACCGGAGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTg                                      <  1:535413/80‑1 (MQ=255)                                       caccacGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGAtt                                   <  1:535415/81‑1 (MQ=255)                                            cGAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAg                              <  1:535416/81‑1 (MQ=255)                                             gAATACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAgg                             >  2:886748/1‑81 (MQ=255)                                              aaTACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGc                            <  2:535417/81‑1 (MQ=255)                                              aaTACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGc                            >  2:535452/1‑81 (MQ=255)                                               aTACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGcc                           >  1:535449/1‑81 (MQ=255)                                                tACCGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCg                          >  1:535457/1‑81 (MQ=255)                                                  ccGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGc                         <  2:535418/80‑1 (MQ=255)                                                   cGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCAc                       >  1:535445/1‑81 (MQ=255)                                                   cGGGGTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCAc                       >  2:535468/1‑81 (MQ=255)                                                      ggTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGcc                    <  2:535419/81‑1 (MQ=255)                                                       gTCTGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCca                   <  2:535420/81‑1 (MQ=255)                                                          tGGACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACAt                >  2:535454/1‑81 (MQ=255)                                                           ggACGTTAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATc               >  1:535458/1‑81 (MQ=255)                                                                ttAGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACa          >  2:535459/1‑81 (MQ=255)                                                                  aGCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACAtt        >  2:535463/1‑81 (MQ=255)                                                                   gCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTc       <  2:535421/81‑1 (MQ=255)                                                                   gCGAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTc       <  2:535422/81‑1 (MQ=255)                                                                     gAAAAAAATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAg     >  1:535471/1‑81 (MQ=255)                                                                        aaaaATACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAgcgc  <  1:535423/81‑1 (MQ=255)                                                                            |                                                                             GCGCGTAATGCGATTAACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGC  >  NC_000913/2894688‑2894839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑