Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,900,805	T→A	F72I (TTT→ATT)	yqcE →	putative transport protein YqcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,900,805	0	T	A	100.0%	62.8 / NA	18	F72I (TTT→ATT)	yqcE	putative transport protein YqcE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/8);  total (10/8)

GTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAATTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCG  >  NC_000913/2900734‑2900882                                                                        |                                                                              gTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACAcc                                                                      <  1:536645/81‑1 (MQ=255)     aataatGAGTACCTTTGGTGTTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCa                                                                   <  1:536646/80‑1 (MQ=255)     aataatGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCa                                                                   >  1:536676/1‑80 (MQ=255)                           gCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCgatgat                                             <  2:536647/80‑1 (MQ=255)                              gCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCAtt                                         <  1:536648/81‑1 (MQ=255)                               ccATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTa                                        <  2:536649/81‑1 (MQ=255)                                 attattCTTTATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTAcc                                      >  1:536672/1‑81 (MQ=255)                                         ttATGCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACt                              >  2:536680/1‑81 (MQ=255)                                             gCCCCCAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGt                          >  1:536683/1‑81 (MQ=255)                                              cccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTc                         <  2:536650/81‑1 (MQ=255)                                               ccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCt                        >  1:536675/1‑81 (MQ=255)                                               ccccAGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCt                        >  2:536674/1‑81 (MQ=255)                                                   aGCGGCGTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTa                    <  1:536651/81‑1 (MQ=255)                                                    gcggcgTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTaa                   >  1:536685/1‑81 (MQ=255)                                                       gcgTTATTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATgg                >  2:536700/1‑81 (MQ=255)                                                            aTTGCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACg           >  1:536691/1‑81 (MQ=255)                                                               gCCGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTAt        >  1:536693/1‑81 (MQ=255)                                                                ccGATAAAATTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATc       <  1:536652/81‑1 (MQ=255)                                                                     aaaaTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCg  >  1:536690/1‑81 (MQ=255)                                                                        |                                                                              GTTTAATAATGAGTACCTTTGGTATTGCGGCCATTATTCTTTATGCCCCCAGCGGCGTTATTGCCGATAAATTTTCACACCGCAAAATGATTACTTCCGCGATGATCATTACCGGATTACTGGGTCTGTTAATGGCAACGTATCCACCG  >  NC_000913/2900734‑2900882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑