Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,906,427	A→G	I239M (ATA→ATG)	ygcG →	protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,427	0	A	G	100.0%	62.3 / NA	18	I239M (ATA→ATG)	ygcG	protein YgcG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/8);  total (10/8)

TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAAC  >  NC_000913/2906352‑2906496                                                                            |                                                                      tctTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGttt                                                                    <  2:537577/79‑1 (MQ=255)           ttATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTAcc                                                        >  1:537597/1‑81 (MQ=255)            tATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTAcc                                                        <  2:537578/80‑1 (MQ=255)               tCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCGCCATGTTTATGGTCTTTACCTGtt                                                    >  2:537602/1‑81 (MQ=255)                ccTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGtt                                                    <  2:537579/80‑1 (MQ=255)                    ttCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTAtgt                                               >  1:537601/1‑81 (MQ=255)                    ttCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTAt                                                 <  1:537580/79‑1 (MQ=255)                           aTAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACtt                                         >  1:537599/1‑80 (MQ=255)                             aaaaTTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACtt                                         >  1:537604/1‑78 (MQ=255)                                       ccATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCAt                            >  1:537608/1‑81 (MQ=255)                                         aTTGCTGTATTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCt                          >  2:537606/1‑81 (MQ=255)                                                 aTTTTCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAg                  >  2:537603/1‑81 (MQ=255)                                                   tttCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGt                 >  1:537609/1‑80 (MQ=255)                                                     tCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTa               <  1:537581/80‑1 (MQ=255)                                                     tCCTTCTTTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTAt              >  1:537605/1‑81 (MQ=255)                                                          cttTTTTACTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCAt           <  2:537582/79‑1 (MQ=255)                                                                 aCTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaa   <  1:537583/80‑1 (MQ=255)                                                                  cTTTCACCATGTTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGacaac  <  2:537584/80‑1 (MQ=255)                                                                            |                                                                      TCTTTTAGATTTATTCCTGTTCTCCGATAAAATTTTTTCCATTGCTGTATTTTCCTTCTTTTTTACTTTCACCATATTTATGGTCTTTACCTGTTTATGTGTACTTCAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAAC  >  NC_000913/2906352‑2906496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑