Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,489	C→T	M151I (ATG→ATA)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,489	0	C	T	100.0%	60.6 / NA	18	M151I (ATG→ATA)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

TTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCA  >  NC_000913/2907412‑2907559                                                                              |                                                                       ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTttat                                                                      >  2:537789/1‑80 (MQ=255)   cgcgCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTtatcat                                                                   >  1:537806/1‑81 (MQ=255)    gcgcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTatcatc                                                                  >  2:537791/1‑81 (MQ=255)    gcgcCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTatcatc                                                                  <  2:537750/81‑1 (MQ=255)        cAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGaa                                                              <  2:537751/81‑1 (MQ=255)         aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAAc                                                             >  2:537783/1‑81 (MQ=255)              gCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                        <  1:537752/81‑1 (MQ=255)                tGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                        >  1:537793/1‑79 (MQ=255)                tGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCa                                                        >  1:537792/1‑79 (MQ=255)                       tGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTAtt                                               >  1:537794/1‑81 (MQ=255)                              cTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCgg                                        <  2:537753/81‑1 (MQ=255)                                    atCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAcc                                  >  1:537800/1‑81 (MQ=255)                                       aaCGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt                               <  2:537754/81‑1 (MQ=255)                                                       tGCTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGc               <  1:537755/81‑1 (MQ=255)                                                         cTCACCACCGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTc             <  2:537756/81‑1 (MQ=255)                                                            accaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGt           <  1:537757/80‑1 (MQ=255)                                                             ccaccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTAc         <  2:537758/81‑1 (MQ=255)                                                              caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa          >  1:537796/1‑79 (MQ=255)                                                              caccGTTGATGATGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACa        <  2:537759/81‑1 (MQ=255)                                                                     gatgatGTTTATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa  <  2:537760/80‑1 (MQ=255)                                                                              |                                                                       TTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCA  >  NC_000913/2907412‑2907559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑