Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,932,902	C→A	D38Y (GAT→TAT)	fucO ←	L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,932,902	0	C	A	100.0%	79.8 / NA	23	D38Y (GAT→TAT)	fucO	L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/11);  total (12/11)

AATCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATCGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAC  >  NC_000913/2932825‑2932978                                                                              |                                                                             aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAgg                                                                            <  1:543042/80‑1 (MQ=255) aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAgg                                                                            <  2:543043/80‑1 (MQ=255) aaTCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGt                                                                           >  1:543084/1‑81 (MQ=255)     gCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGAc                                                                        <  1:543044/80‑1 (MQ=255)     gCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGAc                                                                        >  1:543095/1‑80 (MQ=255)            ccAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAgc                                                                 <  1:543045/80‑1 (MQ=255)             cAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAgcgc                                                               >  1:543085/1‑81 (MQ=255)                cccTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCtt                                                            <  2:543046/81‑1 (MQ=255)                     cagcaTCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGa                                                        >  1:543091/1‑80 (MQ=255)                     cagcaTCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGa                                                        >  2:681625/1‑80 (MQ=255)                           ccatcTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCAc                                                 >  2:543086/1‑81 (MQ=255)                               cTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCg                                             >  2:543088/1‑81 (MQ=255)                                     ggTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCAc                                       <  2:543047/81‑1 (MQ=255)                                                ccaccaCGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGtt                             >  1:543090/1‑80 (MQ=255)                                                ccaccaCGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTa                            <  2:543048/81‑1 (MQ=255)                                                 caccacGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTa                            >  2:543096/1‑80 (MQ=255)                                                    cacGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGc                        >  2:543097/1‑81 (MQ=255)                                                     acGCCGCATTGCACCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGcc                       <  2:543049/81‑1 (MQ=255)                                                                 aCCAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCAccc           <  2:543050/81‑1 (MQ=255)                                                                   cAGCGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCAcccc          >  2:543092/1‑80 (MQ=255)                                                                      cGTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGAcc      <  2:543051/81‑1 (MQ=255)                                                                       gTTTTATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCa     <  1:543052/81‑1 (MQ=255)                                                                          ttATAGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAc  >  1:543093/1‑81 (MQ=255)                                                                              |                                                                             AATCGCCCATGCCAGCCCTGCAGCATCCATCTTATCGGTCACTTTCGCCACCACGCCGCATTGCACCAGCGTTTTATCGGTGACGATCAGCGCCTTCTGATAACCACGGCGTTTCACCTCATCGGTTAAAGCCCCAACAGCACCCCGACCAAAC  >  NC_000913/2932825‑2932978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑