Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	310,270	G→A	G134G (GGC→GGT)	ecpA ←	common pilus major subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	310,270	0	G	A	100.0%	108.7 / NA	30	G134G (GGC→GGT)	ecpA	common pilus major subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/8);  total (22/8)

GATGATGGAGAAGGTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCAC  >  NC_000913/310192‑310347                                                                               |                                                                              gatgatGGAGAAGGTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTacc                                                                             >  1:67644/1‑81 (MQ=255)  gtgatgGAGAAGGTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTaccg                                                                            <  1:67617/80‑1 (MQ=255)  atgatgGAGAAGGTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTacc                                                                             <  2:67616/80‑1 (MQ=255)             ggTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGcc                                                                 <  2:67618/81‑1 (MQ=255)              gTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCg                                                                >  1:67655/1‑81 (MQ=255)               tGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGt                                                               >  1:67668/1‑81 (MQ=255)                gAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGtt                                                              >  2:67673/1‑81 (MQ=255)                   aCCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGc                                                           >  2:67670/1‑81 (MQ=255)                    ccATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCg                                                          >  1:67648/1‑81 (MQ=255)                       tCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTa                                                       >  2:67660/1‑81 (MQ=255)                          tgtgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATc                                                     >  2:67651/1‑80 (MQ=255)                           gtgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGa                                                   >  1:67653/1‑81 (MQ=255)                           gtgCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGa                                                   >  1:67667/1‑81 (MQ=255)                             gCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGat                                                  >  2:67682/1‑80 (MQ=255)                               ggtggtACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGatcat                                               >  1:67654/1‑81 (MQ=255)                                      cGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTa                                        >  2:67650/1‑81 (MQ=255)                                       gATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTAt                                       >  1:67671/1‑81 (MQ=255)                                               gCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAg                                 <  1:67620/79‑1 (MQ=255)                                               gCATTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAg                                 <  2:67619/79‑1 (MQ=255)                                                 aTTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGtttt                             >  2:67664/1‑81 (MQ=255)                                                 aTTGTAACCGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGtttt                             <  1:67621/81‑1 (MQ=255)                                                         cGTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTg                     >  2:67666/1‑81 (MQ=255)                                                          gTTAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGc                    <  2:67622/81‑1 (MQ=255)                                                           ttAGCCAGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGcc                   <  1:67623/81‑1 (MQ=255)                                                             aGCCAGCGGGCTAAGGTTACCGCCCAATACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCcgc                 >  2:67676/1‑81 (MQ=255)                                                                 aGCGGGCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCg             <  1:67624/81‑1 (MQ=255)                                                                     ggCTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTtat         <  2:67625/81‑1 (MQ=255)                                                                      gCTAAGGTTACCGCCCAGTACACCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTtat         >  1:67674/1‑80 (MQ=255)                                                                       cTAAGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATaa       >  2:67677/1‑81 (MQ=255)                                                                         aaGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATc     >  1:67669/1‑81 (MQ=255)                                                                         aaGGTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATc     >  1:67661/1‑81 (MQ=255)                                                                            gTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCAc  >  2:67683/1‑81 (MQ=255)                                                                            gTTACCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCAc  >  2:67685/1‑81 (MQ=255)                                                                               |                                                                              GATGATGGAGAAGGTGAAACCATCCTGTGCGGTGGTACGATTGCTGGCATTGTAACCGTTAGCCAGCGGGCTAAGGTTGCCGCCCAGTACGCCGTTGGCGGTATCGATCATCACGGTATCGCCAGTTTTTTCGACTGCCGCGCCGTTATAATCCAC  >  NC_000913/310192‑310347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑