| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,937,860 | C→T | A131A (GCC→GCT) | fucK → | L‑fuculokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,937,860 | 0 | C | T | 75.0% | 24.0 / 5.8 | 16 | A131A (GCC→GCT) | fucK | L‑fuculokinase |
| Reads supporting (aligned to +/- strand): ref base C (3/1); new base T (6/6); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
GCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCT > NC_000913/2937782‑2937936 | gcgAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCttt < 1:544091/81‑1 (MQ=255) aaCAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAg > 2:544123/1‑81 (MQ=255) gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGttt > 1:544118/1‑80 (MQ=255) gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTc < 1:544092/81‑1 (MQ=255) gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTc < 1:544093/81‑1 (MQ=255) tATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGt > 1:544121/1‑80 (MQ=255) gACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtata > 2:544124/1‑80 (MQ=255) aCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGTtatata < 1:544094/81‑1 (MQ=255) atatTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGTTATATAAGt < 2:544095/81‑1 (MQ=255) aaTCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTga > 2:544126/1‑80 (MQ=255) cGCACAGCGGTTGCAGGCTGTTTCTGGCATCGGAGCTTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa < 2:544096/79‑1 (MQ=255) cGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa < 2:544097/79‑1 (MQ=255) gCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAAct > 2:544133/1‑81 (MQ=255) cTATTTCTGGCGTCGGAGCTTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgctg > 2:544127/1‑80 (MQ=255) tCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgc > 1:544125/1‑81 (MQ=255) cTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgc < 2:544098/80‑1 (MQ=255) gAGCTTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCt > 2:544129/1‑81 (MQ=255) | GCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCT > NC_000913/2937782‑2937936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |