Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,958,495	A→G	Y130Y (TAT→TAC)	ptrA ←	protease 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,958,495	0	A	G	100.0%	109.1 / NA	30	Y130Y (TAT→TAC)	ptrA	protease 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

AGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTC  >  NC_000913/2958424‑2958572                                                                        |                                                                              aGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGcc                                                                      >  2:548587/1‑81 (MQ=255)  gCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCg                                                                     >  1:548560/1‑81 (MQ=255)   cAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGt                                                                    >  2:548571/1‑81 (MQ=255)      aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGc                                                                  >  1:548561/1‑80 (MQ=255)      aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGc                                                                  >  1:548565/1‑80 (MQ=255)      aGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCg                                                                 <  1:548530/81‑1 (MQ=255)            aGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACgg                                                           >  2:548570/1‑81 (MQ=255)            aGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACg                                                            <  1:548531/80‑1 (MQ=255)             gCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGgc                                                          <  2:548532/81‑1 (MQ=255)                   gCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTg                                                    >  2:548582/1‑81 (MQ=255)                     aTCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCt                                                  >  1:548577/1‑81 (MQ=255)                        ggCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGc                                               <  1:548533/81‑1 (MQ=255)                           cAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCAtt                                            <  1:548534/81‑1 (MQ=255)                                ggTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGAc                                       >  2:548566/1‑81 (MQ=255)                                  tCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTa                                     >  1:548581/1‑81 (MQ=255)                                    tACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAcc                                   >  2:548579/1‑81 (MQ=255)                                    tACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAcc                                   >  1:548575/1‑81 (MQ=255)                                     aCCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccg                                  >  1:548580/1‑81 (MQ=255)                                      ccGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccgc                                 <  1:548535/81‑1 (MQ=255)                                       cGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTAccgcc                                <  2:548536/81‑1 (MQ=255)                                              ggCAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtt                         <  2:548537/81‑1 (MQ=255)                                                cAAGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATGCGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt                       >  1:548583/1‑81 (MQ=255)                                                 aaGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt                       >  2:548584/1‑80 (MQ=255)                                                 aaGGCGTCGTTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCAtttt                       >  2:548591/1‑80 (MQ=255)                                                      gtcgtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGata                 >  2:548589/1‑81 (MQ=255)                                                       tcgtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGatat                <  2:548538/81‑1 (MQ=255)                                                         gtTCTCAACTTCCAGGTAGAAAGCCGTGCGATACGGCGCATTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTc              >  1:548576/1‑81 (MQ=255)                                                               aaCTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGcc          <  1:548539/79‑1 (MQ=255)                                                                 cTTCCAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGAc      <  1:548540/81‑1 (MQ=255)                                                                     cAGGTAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTc  <  2:548541/81‑1 (MQ=255)                                                                        |                                                                              AGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCTACCGCACCAGGCAAGGCGTCGTTCTCAACTTCCAGATAGAAAGCCGTGCGATACGGCGCAGTGCTGGCATTGTGACTACCGCCGTGCATTTTGAGATATTCGGCCAGACTGTC  >  NC_000913/2958424‑2958572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑