Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,960,676	C→T	E585K (GAA→AAA)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,960,676	0	C	T	100.0%	149.6 / NA	41	E585K (GAA→AAA)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/19);  total (22/19)

GTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAG  >  NC_000913/2960600‑2960751                                                                             |                                                                            gTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGc                                                                         >  1:549046/1‑81 (MQ=255)    aTCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGa                                                                      <  2:549001/81‑1 (MQ=255)      cgccgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACg                                                                    >  1:549043/1‑81 (MQ=255)       gccgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGc                                                                   >  2:549051/1‑81 (MQ=255)         cgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTc                                                                 <  2:549002/81‑1 (MQ=255)         cgcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTc                                                                 >  2:549041/1‑81 (MQ=255)          gcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTcc                                                                <  1:549003/81‑1 (MQ=255)          gcTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTcc                                                                <  1:549004/81‑1 (MQ=255)           cTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCt                                                               <  2:549005/81‑1 (MQ=255)              cGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCtgtg                                                            >  1:549053/1‑81 (MQ=255)               ggTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGc                                                           >  2:549052/1‑81 (MQ=255)               ggTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGc                                                           >  1:549050/1‑81 (MQ=255)                           cGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACg                                               <  1:549006/81‑1 (MQ=255)                              cAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGc                                              >  2:549063/1‑79 (MQ=255)                                  aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg                                        >  2:549062/1‑81 (MQ=255)                                  aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg                                        <  2:549009/81‑1 (MQ=255)                                  aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg                                        <  2:549007/81‑1 (MQ=255)                                  aagaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATg                                        <  2:549008/81‑1 (MQ=255)                                    gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt                                      >  2:549056/1‑81 (MQ=255)                                    gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt                                      >  1:549045/1‑81 (MQ=255)                                    gaagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtc                                      >  2:549047/1‑80 (MQ=255)                                     aagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGttt                                     <  1:549010/81‑1 (MQ=255)                                     aagGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGtt                                      >  1:549057/1‑80 (MQ=255)                                            tGAGCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCa                              >  2:549064/1‑81 (MQ=255)                                               gCATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCAt                             >  2:549058/1‑79 (MQ=255)                                                cATATCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAg                          >  2:549054/1‑81 (MQ=255)                                                 atatCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAg                          >  1:549055/1‑80 (MQ=255)                                                 atatCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGc                         <  2:549011/81‑1 (MQ=255)                                                    tCGCGACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAg                       <  2:549012/80‑1 (MQ=255)                                                      gcgACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTg                     <  2:549013/80‑1 (MQ=255)                                                       cgACAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTg                     >  2:549074/1‑79 (MQ=255)                                                         aCAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc                 <  2:549014/81‑1 (MQ=255)                                                          cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc                 >  1:549049/1‑80 (MQ=255)                                                          cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc                 >  1:549061/1‑80 (MQ=255)                                                          cAAACCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGc                 >  2:549073/1‑80 (MQ=255)                                                             aCCGGCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGa             <  1:549015/81‑1 (MQ=255)                                                                ggCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGc          <  2:549016/81‑1 (MQ=255)                                                                ggCAACCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGc          >  2:549078/1‑81 (MQ=255)                                                                    aCCACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCcca      <  2:549017/81‑1 (MQ=255)                                                                      cACTCTTTCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCccacc    <  1:549018/81‑1 (MQ=255)                                                                        ctctTTCAGCGGACGCTCCTGTGCCAGCCCGCGACACCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAg  <  1:549019/81‑1 (MQ=255)                                                                             |                                                                            GTCATCGCCGCTTCGGTTTCCGCATCCGGCAGGAAGAAGGCGTTGAGCATATCGCGACAAACCGGCAACCACTCTTCCAGCGGACGCTCCTGTGCCAGCCCGCGACGCCAGATGTTTAGCTGCATTAGCAGTGAAGCCAGATGCCCCACCAG  >  NC_000913/2960600‑2960751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑