Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,343	T→C	V278V (GTT→GTC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,343	0	T	C	100.0%	133.9 / NA	37	V278V (GTT→GTC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

CTGCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAA  >  NC_000913/3008266‑3008420                                                                              |                                                                              cTGCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGc                                                                            >  1:558075/1‑81 (MQ=255)               tgttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGccac                                                              >  2:558098/1‑81 (MQ=255)                   gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcat                                                           >  1:558088/1‑80 (MQ=255)                    aaCCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcatca                                                         >  2:558084/1‑81 (MQ=255)                    aaCCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcatc                                                          <  2:772756/80‑1 (MQ=255)                     aCCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACcatcat                                                        >  1:558085/1‑81 (MQ=255)                          aCAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAg                                                   >  1:558101/1‑81 (MQ=255)                            aaaGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggc                                                 >  1:558097/1‑81 (MQ=255)                              aGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcct                                               <  1:558031/81‑1 (MQ=255)                              aGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcct                                               >  2:558087/1‑81 (MQ=255)                              aGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcct                                               >  1:558082/1‑81 (MQ=255)                                   aCTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAggcctggcct                                          <  2:558032/81‑1 (MQ=255)                                       tATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCtgtg                                       <  1:558034/80‑1 (MQ=255)                                       tATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCtgt                                        <  2:558033/79‑1 (MQ=255)                                         tttATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGc                                     >  1:558104/1‑80 (MQ=255)                                            aTCGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAAc                                 >  2:558099/1‑81 (MQ=255)                                              cGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAAcc                                >  1:558092/1‑80 (MQ=255)                                              cGCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAAcc                                >  2:558094/1‑80 (MQ=255)                                               gCTCCGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTa                              <  2:558035/81‑1 (MQ=255)                                                  ccGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAAcc                           >  1:558091/1‑81 (MQ=255)                                                   cGGCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAAccc                          <  1:558036/81‑1 (MQ=255)                                                     gCGTCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgc                        >  2:558095/1‑81 (MQ=255)                                                        tCAAAGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgg                     <  1:558037/81‑1 (MQ=255)                                                          aaaGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctggg                    <  2:558038/80‑1 (MQ=255)                                                           aaGGCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCgctgggct                  >  1:558100/1‑81 (MQ=255)                                                              gCGACATCGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGctgggctggg               <  2:558039/81‑1 (MQ=255)                                                                     cGTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATcc         >  1:558103/1‑80 (MQ=255)                                                                      gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCt        <  2:558040/80‑1 (MQ=255)                                                                      gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCt        <  1:558041/80‑1 (MQ=255)                                                                      gTCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTa       >  1:558105/1‑81 (MQ=255)                                                                       tCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTAc      <  2:558042/81‑1 (MQ=255)                                                                       tCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTAc      <  2:558044/81‑1 (MQ=255)                                                                       tCAACGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTAc      <  1:558043/81‑1 (MQ=255)                                                                         aaCGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACg     >  1:558118/1‑80 (MQ=255)                                                                          aCGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTTGGCTGGGAAATCCTACGt    >  2:558127/1‑80 (MQ=255)                                                                          aCGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGt    >  2:558093/1‑80 (MQ=255)                                                                           cGTCGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTaa  <  1:558045/81‑1 (MQ=255)                                                                              |                                                                              CTGCACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAA  >  NC_000913/3008266‑3008420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑