Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,863	C→A	I268I (ATC→ATA)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,863	0	C	A	100.0%	104.1 / NA	29	I268I (ATC→ATA)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/15);  total (14/15)

CATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGC  >  NC_000913/3016785‑3016937                                                                               |                                                                           cATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACt                                                                          >  2:559753/1‑81 (MQ=255)  aTGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTc                                                                         <  1:559716/81‑1 (MQ=255)      tGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATg                                                                     >  1:559748/1‑81 (MQ=255)              aaaGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGt                                                             >  2:559766/1‑81 (MQ=255)                       aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTAc                                                    <  2:559717/81‑1 (MQ=255)                          acCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACa                                                   >  1:559754/1‑79 (MQ=255)                          acCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATa                                                 <  2:559718/81‑1 (MQ=255)                            cTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAgg                                               <  2:559719/81‑1 (MQ=255)                              ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCt                                             >  1:559760/1‑81 (MQ=255)                               tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGc                                              <  2:559720/79‑1 (MQ=255)                               tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCt                                             <  1:559721/80‑1 (MQ=255)                                 tgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTa                                           <  2:559722/80‑1 (MQ=255)                                            ccGACCTTACTGGGGTACTCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATATGCTTAAAAGAAGAGTCa                               >  2:559746/1‑81 (MQ=255)                                            ccGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCa                               >  2:559764/1‑81 (MQ=255)                                             cGACCTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAt                              >  1:559765/1‑81 (MQ=255)                                                 cTTACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGa                          >  1:559781/1‑81 (MQ=255)                                                   tACTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGAt                         >  1:559758/1‑80 (MQ=255)                                                    aCTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGAt                         <  2:559723/79‑1 (MQ=255)                                                    aCTGGGGTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATc                        <  1:559724/80‑1 (MQ=255)                                                         ggTACGCGCGTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGAcc                  <  1:559725/81‑1 (MQ=255)                                                              gcgcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg             >  2:559783/1‑81 (MQ=255)                                                               cgcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGc            <  1:559726/81‑1 (MQ=255)                                                                gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt           <  2:559727/81‑1 (MQ=255)                                                                gcgTGTTCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt           <  1:559728/81‑1 (MQ=255)                                                                      tCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc      >  2:559759/1‑80 (MQ=255)                                                                      tCGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc      >  2:559775/1‑80 (MQ=255)                                                                       cGTGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc      >  1:559780/1‑79 (MQ=255)                                                                         tGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc  <  2:559730/81‑1 (MQ=255)                                                                         tGAGATACTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc  <  1:559729/81‑1 (MQ=255)                                                                               |                                                                           CATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGC  >  NC_000913/3016785‑3016937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑