Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,496	A→T	E479D (GAA→GAT)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,496	0	A	T	100.0%	143.9 / NA	40	E479D (GAA→GAT)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/23);  total (17/23)

ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC  >  NC_000913/3017420‑3017572                                                                             |                                                                             aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc                                                                          <  1:559852/81‑1 (MQ=255) aCTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCAcc                                                                          <  2:559851/81‑1 (MQ=255)     cTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTa                                                                      >  2:559903/1‑81 (MQ=255)         gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCTTAATACATCCGTCTGCTTGGCGATCACCGCTATGcc                                                                  >  1:559914/1‑81 (MQ=255)         gTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGcc                                                                  <  1:559853/81‑1 (MQ=255)          ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg                                                                 <  2:559855/81‑1 (MQ=255)          ttGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCg                                                                 <  1:559854/81‑1 (MQ=255)           tGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGa                                                                >  2:559909/1‑81 (MQ=255)            gCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGa                                                                <  1:559856/80‑1 (MQ=255)            gCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc                                                               >  2:559912/1‑81 (MQ=255)             cccccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAc                                                               <  1:559857/80‑1 (MQ=255)               cccTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGAcgcg                                                            >  1:559900/1‑81 (MQ=255)                 cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt                                                          <  1:559858/81‑1 (MQ=255)                 cTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCt                                                          <  2:559859/81‑1 (MQ=255)                   gtgtTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTg                                                         >  2:559920/1‑80 (MQ=255)                      ttACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAAc                                                     <  1:559860/81‑1 (MQ=255)                        aCTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACt                                                    >  2:559916/1‑80 (MQ=255)                         ctgcctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTc                                                   <  2:559861/80‑1 (MQ=255)                            cctgcGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCAt                                                 <  1:559862/79‑1 (MQ=255)                                     tCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgc                                       <  2:559863/80‑1 (MQ=255)                                     tCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacg                                        <  2:559864/79‑1 (MQ=255)                                       aagcaagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACcaacgcaa                                     <  2:559865/80‑1 (MQ=255)                                           aagATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGctc                                <  1:559866/81‑1 (MQ=255)                                            agATATTCCGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGctct                               <  2:559867/81‑1 (MQ=255)                                                   ccGGAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGc                         <  2:559868/80‑1 (MQ=255)                                                     ggAATACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCAt                      >  1:559924/1‑81 (MQ=255)                                                        aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc                   >  2:559927/1‑81 (MQ=255)                                                        aTACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAc                   >  2:559919/1‑81 (MQ=255)                                                         tACATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTAcc                  <  1:559869/81‑1 (MQ=255)                                                          aCATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCg                 >  2:559953/1‑81 (MQ=255)                                                           cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCgg                >  1:559918/1‑81 (MQ=255)                                                           cATCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCgg                >  1:559915/1‑81 (MQ=255)                                                             tCCGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTc              <  1:559870/81‑1 (MQ=255)                                                              ccGTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCa             >  2:559917/1‑81 (MQ=255)                                                                gTCTGCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCata           >  1:559911/1‑81 (MQ=255)                                                                    gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg       >  2:559922/1‑81 (MQ=255)                                                                    gCTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTg       >  1:559913/1‑81 (MQ=255)                                                                     cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt        <  1:559871/79‑1 (MQ=255)                                                                     cTTGGCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATAttt        <  2:559872/79‑1 (MQ=255)                                                                         gCGATCACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATc  <  2:559873/81‑1 (MQ=255)                                                                             |                                                                             ACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGATATTCCGGAATACATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATC  >  NC_000913/3017420‑3017572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑