Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,867	G→A	G725G (GGC→GGT)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,867	0	G	A	100.0%	117.0 / NA	33	G725G (GGC→GGT)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943                                                                               |                                                                             cgcgCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACAcc                                                                            <  2:566486/81‑1 (MQ=255)  gcgcCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACAcc                                                                            >  2:566522/1‑80 (MQ=255)    gcCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGAt                                                                         <  1:566487/81‑1 (MQ=255)     cccTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATc                                                                        >  2:566537/1‑81 (MQ=255)          aCGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTcc                                                                   <  2:566488/81‑1 (MQ=255)           cGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTccc                                                                  <  1:566489/81‑1 (MQ=255)                 aaCATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATAccc                                                            >  2:566533/1‑81 (MQ=255)                           cGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccacc                                                    <  1:566490/79‑1 (MQ=255)                            gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 <  2:566491/81‑1 (MQ=255)                            gATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGaccaccacc                                                 >  1:566535/1‑81 (MQ=255)                                tGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              <  2:566492/80‑1 (MQ=255)                                tGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCAccgcc                                              <  1:566493/80‑1 (MQ=255)                                 gCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGt                                            >  1:566544/1‑81 (MQ=255)                                  caccacGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTg                                           <  1:566495/81‑1 (MQ=255)                                  caccacGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTg                                           <  1:566494/81‑1 (MQ=255)                                    ccacGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCg                                         <  1:566496/81‑1 (MQ=255)                                      acGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGa                                       <  1:905879/81‑1 (MQ=255)                                        gcaaTGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAAt                                     <  1:566497/78‑1 (MQ=255)                                        gCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAAt                                     <  1:566498/81‑1 (MQ=255)                                        gCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAAt                                     >  2:566540/1‑81 (MQ=255)                                         cTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATg                                    >  1:566543/1‑81 (MQ=255)                                         cTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATg                                    <  2:566499/81‑1 (MQ=255)                                           aTGACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATg                                    >  2:566542/1‑79 (MQ=255)                                             gACCCGGTACAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAg                                 >  2:566534/1‑80 (MQ=255)                                                      cAAACGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTa                        <  2:566500/80‑1 (MQ=255)                                                       aaaCGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATg                      >  2:566562/1‑81 (MQ=255)                                                          cGGTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAg                   <  2:566501/81‑1 (MQ=255)                                                           ggTGCCAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAgg                  <  2:566502/81‑1 (MQ=255)                                                              tacAAATGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTa               <  2:566503/79‑1 (MQ=255)                                                                 aaaTGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAgg            >  2:566550/1‑81 (MQ=255)                                                                    tGCGCTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGgtgt         <  2:566504/81‑1 (MQ=255)                                                                       gcTTTCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGaa      <  2:566505/81‑1 (MQ=255)                                                                           tCACACCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGt  >  2:566561/1‑81 (MQ=255)                                                                               |                                                                             CGCGCCCTGACGGGTTAACATGCCTTCGATTTGCACCACGCTATGACCCGGTACAAACGGTGCCAAATGCGCTTTCACGCCGATCGGTCCCATACCCGGACCACCACCGCCGTGCGGAATGCAGAAAGTTTTATGTAGGTTAAGGTGTGAAACGT  >  NC_000913/3046789‑3046943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑