Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,073,292	C→A	V134F (GTT→TTT)	epd ←	D‑erythrose‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,073,292	0	C	A	100.0%	173.4 / NA	47	V134F (GTT→TTT)	epd	D‑erythrose‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

AATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAT  >  NC_000913/3073217‑3073369                                                                            |                                                                              aaTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcg                                                                          >  2:572532/1‑81 (MQ=255)  aTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgc                                                                         <  1:572473/81‑1 (MQ=255)  aTTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgc                                                                         <  2:572472/81‑1 (MQ=255)   ttCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTcgcg                                                                        <  1:572474/81‑1 (MQ=255)      gTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTc                                                                      >  1:572529/1‑80 (MQ=255)       tGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTc                                                                      >  1:572550/1‑79 (MQ=255)            cAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATc                                                                <  1:572475/80‑1 (MQ=255)            cAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCg                                                               >  2:572525/1‑81 (MQ=255)             aGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGt                                                              >  1:572534/1‑81 (MQ=255)               gAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTa                                                            >  2:572522/1‑81 (MQ=255)               gAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTa                                                            >  1:572521/1‑81 (MQ=255)                aaGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTAc                                                           >  1:572552/1‑81 (MQ=255)                 aGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACt                                                          <  1:572476/81‑1 (MQ=255)                    gTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGc                                                        >  1:572558/1‑80 (MQ=255)                    gTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGc                                                        >  2:572537/1‑80 (MQ=255)                     ttAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCa                                                      <  2:572477/81‑1 (MQ=255)                        gAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGa                                                   >  1:572548/1‑81 (MQ=255)                         aaaCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAt                                                  >  1:572526/1‑81 (MQ=255)                         aaaCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAt                                                  <  1:572478/81‑1 (MQ=255)                         aaaCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAt                                                  >  2:572524/1‑81 (MQ=255)                          aaCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtg                                                 >  2:572531/1‑81 (MQ=255)                           aCGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtgt                                                >  2:572549/1‑81 (MQ=255)                            cGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGAtgtg                                               <  2:572479/81‑1 (MQ=255)                              aTGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGaa                                             <  2:572480/81‑1 (MQ=255)                                gCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGaa                                             >  1:572539/1‑79 (MQ=255)                                  ggTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAaga                                         <  1:572482/81‑1 (MQ=255)                                  ggTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAaga                                         <  2:572481/81‑1 (MQ=255)                                   gtgtTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAagag                                        <  2:572483/81‑1 (MQ=255)                                      ttCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCAc                                     <  1:572484/81‑1 (MQ=255)                                          gCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtttt                                 <  2:572485/81‑1 (MQ=255)                                          gCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtttt                                 >  2:572533/1‑81 (MQ=255)                                          gCACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtttt                                 <  1:572486/81‑1 (MQ=255)                                           cACGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACttttt                                <  1:572487/81‑1 (MQ=255)                                            aCGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACtttttt                               <  1:572488/81‑1 (MQ=255)                                             cGAAGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTg                              <  1:572489/81‑1 (MQ=255)                                                aGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGcc                           <  2:572490/81‑1 (MQ=255)                                                aGTTGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGcc                           <  2:572491/81‑1 (MQ=255)                                                  ttGATCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGcccc                         >  2:572555/1‑81 (MQ=255)                                                     aTCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcgg                       <  1:572492/80‑1 (MQ=255)                                                      tCCTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcggcg                     <  2:572493/81‑1 (MQ=255)                                                       ccTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcggcgg                    >  2:572559/1‑81 (MQ=255)                                                       ccTGATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCcggcgg                    >  1:572564/1‑81 (MQ=255)                                                          gATTGACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCaa                 >  2:572554/1‑81 (MQ=255)                                                              gACGCCGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATg             >  2:572562/1‑81 (MQ=255)                                                                  ccGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCt         <  1:572494/81‑1 (MQ=255)                                                                   cGTAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCtt        >  1:572536/1‑81 (MQ=255)                                                                     tAAACAAAGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCg      >  2:572556/1‑81 (MQ=255)                                                                       aacaaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGcc    <  2:572496/81‑1 (MQ=255)                                                                       aacaaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGcc    <  2:572495/81‑1 (MQ=255)                                                                       aacaaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGc     >  1:572545/1‑80 (MQ=255)                                                                         caaaGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAt  <  1:572497/81‑1 (MQ=255)                                                                            |                                                                              AATTCGTGGTACAGGAAGCGTTAGAAACGATGCGGTGTTCCGCACGAAGTTGATCCTGATTGACGCCGTAAACAACGGTCGCGTCGAGATCGTTACTGCCAGGATGTGAAAAGAGCACTTTTTTGGCCCCGGCGGCAATATGCGCTTCGCCAT  >  NC_000913/3073217‑3073369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑