Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,659	C→T	A401T (GCA→ACA)	cmtA ←	mannitol‑specific PTS enzyme IICB component CmtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,659	0	C	T	100.0%	77.3 / NA	22	A401T (GCA→ACA)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

CCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACA  >  NC_000913/3077584‑3077735                                                                            |                                                                             ccAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGc                                                                          <  2:573460/80‑1 (MQ=255)  cAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGccc                                                                        <  2:573461/81‑1 (MQ=255)        ggCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCttt                                                                  >  2:573495/1‑81 (MQ=255)         gCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCttt                                                                  <  2:573462/80‑1 (MQ=255)           aTGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTc                                                               >  1:573491/1‑81 (MQ=255)           aTGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTc                                                               >  2:573501/1‑81 (MQ=255)                    gacgaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGtt                                                       >  1:573504/1‑80 (MQ=255)                    gacgaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGtt                                                       >  2:573500/1‑80 (MQ=255)                       gaTATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACg                                                   >  2:573502/1‑81 (MQ=255)                           tccgcatccgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGaa                                                <  1:573463/80‑1 (MQ=255)                                  ccgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcg                                        >  2:573509/1‑81 (MQ=255)                                   cgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcg                                        <  2:573465/80‑1 (MQ=255)                                   cgcaGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCACCGCTTTTTCCAGGCGTTTACGGAAGGTGGTcgc                                       <  1:573464/81‑1 (MQ=255)                                       ggCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGcc                                    >  2:573510/1‑80 (MQ=255)                                             ttttCTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCa                             <  1:573466/81‑1 (MQ=255)                                                tCTATGGCGTAATGTTTTACTTCAATTGTCGGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACt                           <  2:573467/80‑1 (MQ=255)                                                 cTATGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTg                          >  2:573505/1‑80 (MQ=255)                                                    tGGCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAg                       <  1:573468/80‑1 (MQ=255)                                                      gCGTAATGTTTTACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGccc                    <  2:573469/81‑1 (MQ=255)                                                               tttACTTCAATTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCa           >  1:573513/1‑81 (MQ=255)                                                                        aTTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGcaaaca  <  2:573470/81‑1 (MQ=255)                                                                        aTTGTCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGcaaac   >  1:573521/1‑80 (MQ=255)                                                                            |                                                                             CCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACA  >  NC_000913/3077584‑3077735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑