Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	349,262	C→T	A194V (GCC→GTC)	prpB →	2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	349,262	0	C	T	100.0%	113.5 / NA	32	A194V (GCC→GTC)	prpB	2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/14);  total (18/14)

CGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCT  >  NC_000913/349187‑349337                                                                            |                                                                            cggcgATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATg                                                                         <  2:74632/80‑1 (MQ=255) cggcgATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATg                                                                         >  2:74693/1‑80 (MQ=255)  ggcgATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTa                                                                       >  1:74678/1‑81 (MQ=255)    cgATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTAcc                                                                     >  2:74680/1‑81 (MQ=255)      aTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGc                                                                   <  2:74633/81‑1 (MQ=255)      aTCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGc                                                                   >  1:74697/1‑81 (MQ=255)       tCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGc                                                                   >  1:74704/1‑80 (MQ=255)        cGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGc                                                                   >  1:74694/1‑79 (MQ=255)         gAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCa                                                                 <  1:74634/80‑1 (MQ=255)          aGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGt                                                               <  2:74635/81‑1 (MQ=255)           gCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGtt                                                              <  1:74636/81‑1 (MQ=255)                  cAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGAt                                                       >  1:74677/1‑81 (MQ=255)                       cTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGt                                                  <  1:74637/81‑1 (MQ=255)                         aTGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTg                                                 >  2:74699/1‑80 (MQ=255)                               aaGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTg                                           <  2:74638/80‑1 (MQ=255)                                aGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGcc                                         >  2:74690/1‑81 (MQ=255)                                   gggTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGAt                                      >  1:74702/1‑81 (MQ=255)                                   gggTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGAt                                      <  1:74639/81‑1 (MQ=255)                                    ggTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATc                                     >  1:74698/1‑81 (MQ=255)                                     gTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATcc                                    >  1:74687/1‑81 (MQ=255)                                      tGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCt                                   <  1:74640/81‑1 (MQ=255)                                             aTGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCGGATCCTCGCCAAc                            <  2:74641/81‑1 (MQ=255)                                             aTGTTGTTCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCaa                             >  1:74711/1‑80 (MQ=255)                                                ttgttCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACAt                          <  2:74642/80‑1 (MQ=255)                                                  gttCCCGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCAc                       >  2:74710/1‑81 (MQ=255)                                                     cccGGAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCg                     >  1:74695/1‑80 (MQ=255)                                                        ggAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAAtt                 <  1:74643/81‑1 (MQ=255)                                                         gAGGCGATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAAttt                >  1:74721/1‑81 (MQ=255)                                                              gATTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGc           >  1:74709/1‑81 (MQ=255)                                                               aTTACCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGcc          <  2:74644/81‑1 (MQ=255)                                                                  aCCGAACTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCAc        <  2:74645/80‑1 (MQ=255)                                                                       aCTCGTCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCt  >  2:74713/1‑81 (MQ=255)                                                                            |                                                                            CGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTTCCCGGAGGCGATTACCGAACTCGCCATGTACCGCCAGTTTGCCGATGCGGTGCAGGTGCCGATCCTCGCCAACATCACCGAATTTGGTGCCACGCCGCT  >  NC_000913/349187‑349337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑