Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,585	C→T	A101V (GCT→GTT)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,585	0	C	T	100.0%	93.8 / NA	26	A101V (GCT→GTT)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

AAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGC  >  NC_000913/3088515‑3088660                                                                       |                                                                            aaaCTCGGGCGCAAAAAGAGCCTCATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAAc                                                                   >  2:576127/1‑81 (MQ=255)      cGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTg                                                               >  2:576124/1‑80 (MQ=255)             aaaaaGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACt                                                        >  2:576132/1‑80 (MQ=255)                   agCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCtt                                                 >  2:576130/1‑81 (MQ=255)                   agCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCtt                                                 >  2:576123/1‑81 (MQ=255)                    gCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCttt                                                <  1:576088/81‑1 (MQ=255)                      cTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTcc                                              <  2:576089/81‑1 (MQ=255)                      cTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTcc                                              >  1:576136/1‑81 (MQ=255)                         atgatCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCcg                                            <  2:576090/80‑1 (MQ=255)                            atCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGtt                                        >  2:576137/1‑81 (MQ=255)                            atCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGtt                                        >  1:576135/1‑81 (MQ=255)                               ggCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCt                                      <  2:576092/80‑1 (MQ=255)                               ggCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCt                                      <  1:576091/80‑1 (MQ=255)                                gcgcAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTAc                                    <  2:576093/81‑1 (MQ=255)                                    aaTTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTggg                                <  2:576094/81‑1 (MQ=255)                                     aTTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTgggg                               >  1:576129/1‑81 (MQ=255)                                       tttgtttgTTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCACGCGTTCTACTGGGGCt                             >  1:576148/1‑81 (MQ=255)                                        ttgtttgtTGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCt                             <  2:576095/80‑1 (MQ=255)                                           tttgttGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGc                          <  1:576097/80‑1 (MQ=255)                                           tttgttGCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCg                         <  2:576096/81‑1 (MQ=255)                                            ttgttgCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCgg                        >  1:576131/1‑81 (MQ=255)                                               ttgCCGGTTCGCTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCggtg                      <  1:576098/80‑1 (MQ=255)                                                          cTGTTCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCctct          <  1:576099/81‑1 (MQ=255)                                                             ttCTCTGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTta        <  1:576100/80‑1 (MQ=255)                                                               ctctGCGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATac     >  1:576150/1‑81 (MQ=255)                                                                    cGGTTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATaccgc  <  1:576101/79‑1 (MQ=255)                                                                       |                                                                            AAACTCGGGCGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCGGCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGTGTGGCCTCTTATACCGC  >  NC_000913/3088515‑3088660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑