Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,094,484	G→A	L199L (CTC→CTT)	yggR ←	Type II/IV secretion system family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,094,484	0	G	A	100.0%	135.4 / NA	37	L199L (CTC→CTT)	yggR	Type II/IV secretion system family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

AATGTTGCCAGCACCAAATGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCGAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGTGCAAACCAAT  >  NC_000913/3094407‑3094558                                                                              |                                                                           aaTGTTGCCAGCACCAAATGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAg                                                                          >  2:577522/1‑80 (MQ=255)  aTGTTGCCAGCACCAAATGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGc                                                                         <  1:913873/80‑1 (MQ=255)               cAAATGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAg                                                            >  2:577533/1‑80 (MQ=255)                 aaTGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGAt                                                         >  2:577526/1‑81 (MQ=255)                  aTGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATc                                                        >  2:577578/1‑81 (MQ=255)                  aTGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATc                                                        >  1:577536/1‑81 (MQ=255)                   tGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCt                                                       >  2:577534/1‑81 (MQ=255)                    gCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCtt                                                      >  2:577524/1‑81 (MQ=255)                     cccGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTc                                                     <  2:577475/81‑1 (MQ=255)                      ccGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTcc                                                    <  1:577476/81‑1 (MQ=255)                      ccGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTcc                                                    >  2:577535/1‑81 (MQ=255)                             cTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAAt                                              <  1:577478/80‑1 (MQ=255)                             cTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAAt                                              <  2:577477/80‑1 (MQ=255)                                 cgccgTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGc                                         <  2:577479/81‑1 (MQ=255)                                  gccgTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGcc                                        >  1:577525/1‑81 (MQ=255)                                  gccgTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGcc                                        >  2:577543/1‑81 (MQ=255)                                    cgTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCg                                      >  1:577537/1‑81 (MQ=255)                                     gTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCg                                      >  1:577542/1‑80 (MQ=255)                                     gTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGc                                     <  1:577480/81‑1 (MQ=255)                                       cAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCaa                                   >  2:577545/1‑81 (MQ=255)                                             cAGACGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGa                             >  1:577481/1‑81 (MQ=255)                                                aCGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGc                          >  1:577538/1‑81 (MQ=255)                                                aCGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGc                          <  1:577482/81‑1 (MQ=255)                                                 cGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGc                          >  1:577527/1‑80 (MQ=255)                                                 cGGATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCg                         <  1:577483/81‑1 (MQ=255)                                                  ggATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGa                        <  2:577485/81‑1 (MQ=255)                                                  ggATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGa                        <  1:577484/81‑1 (MQ=255)                                                   gATTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGaa                       <  1:577486/81‑1 (MQ=255)                                                    aTTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAAc                      <  1:577487/81‑1 (MQ=255)                                                    aTTGTCTCGCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAAc                      >  1:577529/1‑81 (MQ=255)                                                            gCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATa                >  2:577530/1‑79 (MQ=255)                                                            gCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATa                <  1:577488/79‑1 (MQ=255)                                                            gCTGTCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACa              <  1:577489/81‑1 (MQ=255)                                                                tCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGt            <  1:577490/79‑1 (MQ=255)                                                                tCACGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGTg           >  2:577539/1‑80 (MQ=255)                                                                  aCGCAGCTCTCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGTGCaa        <  1:577491/81‑1 (MQ=255)                                                                        ctctCCAAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGTGCAAACCAAt  <  2:577492/81‑1 (MQ=255)                                                                              |                                                                           AATGTTGCCAGCACCAAATGCCCGGTTTCTGCCGCCGTCAGTGCCAGACGGATTGTCTCGCTGTCACGCAGCTCTCCGAGCAAAATCACATCAGGATCTTCCCGCAATGCGGCCCGCAATCCCGATGCGAACGTCATACAGTGCAAACCAAT  >  NC_000913/3094407‑3094558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑