| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,121,875 | T→C | N644S (AAC→AGC) | glcB ← | malate synthase G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,121,875 | 0 | T | C | 95.8% | 77.2 / ‑3.7 | 24 | N644S (AAC→AGC) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (11/12); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTT > NC_000913/3121800‑3121952 | tGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGc > 2:583522/1‑80 (MQ=255) aaCCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGatat < 1:583479/80‑1 (MQ=255) aCTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATgctgg < 2:583480/81‑1 (MQ=255) cTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATgctggc > 2:583510/1‑81 (MQ=255) cATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGAt < 1:583481/81‑1 (MQ=255) tctcCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACg > 2:583509/1‑79 (MQ=255) aGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGtt < 2:583482/81‑1 (MQ=255) gCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTg > 2:583539/1‑81 (MQ=255) gCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTg > 1:583516/1‑81 (MQ=255) gACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTgcg > 1:583519/1‑81 (MQ=255) gCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCgg > 1:583515/1‑81 (MQ=255) tGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCt > 1:583568/1‑81 (MQ=255) gCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCtt > 1:583520/1‑81 (MQ=255) ccTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCa < 2:583483/81‑1 (MQ=255) cTTTGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAAc < 1:583484/80‑1 (MQ=255) tttGGTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGc > 1:583511/1‑81 (MQ=255) ggTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCca > 1:583524/1‑80 (MQ=255) gTCAGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCcaca < 1:583485/81‑1 (MQ=255) aGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACAtt < 1:583487/80‑1 (MQ=255) aGAATACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACAtt < 2:583486/80‑1 (MQ=255) aTACCGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGa < 2:583488/81‑1 (MQ=255) cGTGACGTAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAatat < 2:583489/81‑1 (MQ=255) tAACCAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCAc < 2:583490/81‑1 (MQ=255) cAGCTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCACtttt < 2:583491/81‑1 (MQ=255) | TGATCAACCACTTTCGCCATATTCTCCAGCGACGCCTGCACCTGTTCTTTGGTCAGAATACCGTGACGTAACCAGTTGGCGATATGCTGGCTGGAGATACGCAGCGTTGCGCGGTCTTCCATCAACGCCACATTGTGAATATCCGGCACTTTT > NC_000913/3121800‑3121952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |