| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,142,898 | G→A | A31V (GCA→GTA) | hybC ← | hydrogenase 2 large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,142,898 | 0 | G | A | 97.4% | 136.0 / ‑3.6 | 38 | A31V (GCA→GTA) | hybC | hydrogenase 2 large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/26); minor base T (0/1); total (11/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
ACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAA > NC_000913/3142820‑3142970 | acaATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACt < 1:588429/81‑1 (MQ=255) atcatcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTc < 1:588430/81‑1 (MQ=255) atcatcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTc < 2:588431/81‑1 (MQ=255) atcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGa < 1:588433/80‑1 (MQ=255) atcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGa < 2:588432/80‑1 (MQ=255) aTGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAacga < 2:588434/81‑1 (MQ=255) gCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAacgacg > 1:588496/1‑81 (MQ=255) aTCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGcc < 1:588435/81‑1 (MQ=255) aTCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGc > 2:588487/1‑80 (MQ=255) gcgcgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCAtt < 2:588437/81‑1 (MQ=255) gcgcgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCAtt < 2:588436/81‑1 (MQ=255) cgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTc < 2:588438/81‑1 (MQ=255) cgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTc < 1:588440/81‑1 (MQ=255) cgGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTc < 1:588439/81‑1 (MQ=255) aTCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGAt < 1:588441/81‑1 (MQ=255) cgGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCg < 2:588442/81‑1 (MQ=255) ggTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATTCTTTCGAAACGACGCCATTTTCGATTTCGc < 1:588443/81‑1 (MQ=255) gTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGc > 1:588507/1‑80 (MQ=255) ttCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATc > 2:588489/1‑80 (MQ=255) tCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCg < 1:588444/80‑1 (MQ=255) tCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGa > 1:588493/1‑81 (MQ=255) aTCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCg > 1:588494/1‑80 (MQ=255) aTCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCg > 1:588513/1‑80 (MQ=255) ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGt < 2:588447/76‑1 (MQ=255) ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGt < 1:588445/76‑1 (MQ=255) ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTaaa < 2:588446/79‑1 (MQ=255) ttCCATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTa < 2:588448/77‑1 (MQ=255) ccATGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGcc > 2:588501/1‑81 (MQ=255) aTGCCGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGcccc < 1:588449/81‑1 (MQ=255) ccGCGCCACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTc < 2:588450/80‑1 (MQ=255) ccACATGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACg < 2:588451/81‑1 (MQ=255) caTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGt < 1:588452/81‑1 (MQ=255) caTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGt < 1:588453/81‑1 (MQ=255) aTGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGtt < 1:588454/81‑1 (MQ=255) tGGTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTa < 2:588455/81‑1 (MQ=255) gTACCGGAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTAcc > 2:588511/1‑81 (MQ=255) ggAAGCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGAt > 1:588504/1‑80 (MQ=255) gCCCATACTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCaa > 1:588456/1‑79 (MQ=255) | ACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAA > NC_000913/3142820‑3142970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |