Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,149,148	T→C	K108K (AAA→AAG)	yqhA ←	uncharacterized protein YqhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,149,148	0	T	C	100.0%	103.4 / NA	29	K108K (AAA→AAG)	yqhA	uncharacterized protein YqhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

ATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGT  >  NC_000913/3149073‑3149223                                                                            |                                                                            aTCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGtt                                                                        >  2:589969/1‑81 (MQ=255)    cGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGttttt                                                                     >  2:589980/1‑81 (MQ=255)    cGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGttttt                                                                     >  1:589970/1‑81 (MQ=255)              ttCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGt                                                            <  1:589926/80‑1 (MQ=255)               tCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGtt                                                           <  1:589927/80‑1 (MQ=255)               tCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGtt                                                           <  2:589928/80‑1 (MQ=255)                   aTCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTc                                                      >  2:589975/1‑81 (MQ=255)                    tCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTcc                                                     <  2:589929/81‑1 (MQ=255)                       aTAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGTTGCTACCTTGTTTTCCAGCGACGTTGTGTCCAtt                                                  >  1:589967/1‑81 (MQ=255)                         aaaGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCAtttt                                                >  1:589966/1‑81 (MQ=255)                            gACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTccc                                             >  1:589971/1‑81 (MQ=255)                              cgcgCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTccc                                             >  1:589991/1‑79 (MQ=255)                                 gcAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCccagc                                         >  1:589972/1‑80 (MQ=255)                                       aGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTc                                   <  2:589930/80‑1 (MQ=255)                                           aaTGGAAGAAATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCtt                              <  1:589931/81‑1 (MQ=255)                                                 agaaATTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCttt                        <  2:589932/81‑1 (MQ=255)                                                   aaaTTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGt                      <  1:589933/81‑1 (MQ=255)                                                    aaTTGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGtt                     >  1:589981/1‑81 (MQ=255)                                                       tGCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTtctc                  >  1:589992/1‑81 (MQ=255)                                                        gCCACAATCGCCGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCg                 >  2:589993/1‑81 (MQ=255)                                                        gCCACAATCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTtctc                  <  1:589934/80‑1 (MQ=255)                                                             aaTCGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGat            <  2:589935/81‑1 (MQ=255)                                                                cGACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATc         >  2:589988/1‑81 (MQ=255)                                                                 gACGCTGCTACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATcc        <  1:589936/81‑1 (MQ=255)                                                                    gctgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGc     >  1:589977/1‑81 (MQ=255)                                                                    gctgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCCTCTCTTTGTTCTCGGAGATATCCAGc     >  2:589982/1‑81 (MQ=255)                                                                      tgctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtg   >  2:589989/1‑81 (MQ=255)                                                                       gctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtgt  >  2:589976/1‑81 (MQ=255)                                                                        ctACCTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCtg   <  1:589937/79‑1 (MQ=255)                                                                            |                                                                            ATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGCCACAATCGACGCTGCTACTTTGTTTTTCAGCGACGTTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGT  >  NC_000913/3149073‑3149223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑