Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,152,036	C→T	intergenic (‑52/‑200)	exbB ← / → metC	Ton complex subunit ExbB/cystathionine beta‑lyase/L‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,152,036	0	C	T	100.0%	108.0 / NA	30	intergenic (‑52/‑200)	exbB/metC	Ton complex subunit ExbB/cystathionine beta‑lyase/L‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/18);  total (12/18)

GTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCTT  >  NC_000913/3151958‑3152102                                                                               |                                                                   gTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAatga                                                                  >  2:590665/1‑81 (MQ=255)     gTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTc                                                              <  2:590615/81‑1 (MQ=255)     gTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTc                                                              >  2:590666/1‑81 (MQ=255)     gTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTc                                                              >  1:590669/1‑81 (MQ=255)      tCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCg                                                             <  1:590616/81‑1 (MQ=255)       cTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCg                                                             >  1:590659/1‑80 (MQ=255)            ttAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGa                                                       >  2:590664/1‑81 (MQ=255)              aaaTTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGAt                                                      <  1:590617/80‑1 (MQ=255)                attattACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGt                                                   <  2:590618/81‑1 (MQ=255)                   attaCCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCg                                                 <  1:590619/80‑1 (MQ=255)                    ttaCCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGt                                                <  2:590620/80‑1 (MQ=255)                     taCCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTtc                                              >  2:590660/1‑81 (MQ=255)                        ccACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCa                                           >  2:590662/1‑81 (MQ=255)                        ccACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCa                                           <  1:590621/81‑1 (MQ=255)                         cACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCAt                                          <  1:590622/81‑1 (MQ=255)                             gtgtATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTAc                                       >  2:590667/1‑80 (MQ=255)                             gtgtATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTAc                                       >  2:590656/1‑80 (MQ=255)                                     ccAGAACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCa                               >  1:590671/1‑80 (MQ=255)                                      cAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTg                                 <  1:590623/77‑1 (MQ=255)                                      cAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTg                                 <  2:590624/77‑1 (MQ=255)                                       aGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATAc                            >  1:590672/1‑81 (MQ=255)                                        ggACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACt                           <  2:590625/81‑1 (MQ=255)                                        ggACGCAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACt                           >  2:590661/1‑81 (MQ=255)                                             cAAGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGt                      <  1:590626/81‑1 (MQ=255)                                               aGTCACAAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTAc                    <  2:590627/81‑1 (MQ=255)                                                   acaAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCtt                 <  1:590629/80‑1 (MQ=255)                                                   acaAAATCTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCtt                 <  1:590628/80‑1 (MQ=255)                                                          cTGCGCATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttct         >  2:590670/1‑81 (MQ=255)                                                             cgcATAATAATATCAAAATGACGTCGAATTGATAGTTGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttc          <  1:590631/77‑1 (MQ=255)                                                             cgcATAATAATATCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttctt        <  1:590630/79‑1 (MQ=255)                                                                 taataataTCAAAATGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCtt  <  1:590632/81‑1 (MQ=255)                                                                               |                                                                   GTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCTT  >  NC_000913/3151958‑3152102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑