Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,152,994	C→T	S253S (AGC→AGT)	metC →	cystathionine beta‑lyase/L‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,152,994	0	C	T	100.0%	134.7 / NA	37	S253S (AGC→AGT)	metC	cystathionine beta‑lyase/L‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

TTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCT  >  NC_000913/3152919‑3153065                                                                            |                                                                        ttGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTgg                                                                    >  2:590879/1‑81 (MQ=255)      gggAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgc                                                               >  1:590890/1‑81 (MQ=255)        gAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgcgc                                                             <  2:590837/81‑1 (MQ=255)        gAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTgcg                                                              >  2:590875/1‑80 (MQ=255)          gcagcTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGcac                                                           <  2:590838/81‑1 (MQ=255)           cagcTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGcaca                                                          >  2:590880/1‑81 (MQ=255)                aCGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAgg                                                     >  2:590872/1‑81 (MQ=255)                 cGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAg                                                      <  1:590839/79‑1 (MQ=255)                   ggAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgt                                                  >  2:590882/1‑81 (MQ=255)                   ggAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgt                                                  >  1:590888/1‑81 (MQ=255)                    gAAAATGCCTATCTGATGAGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGtgtg                                                 <  1:590841/81‑1 (MQ=255)                    gAAAATGCCTATCTGATCTGCCTGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGgtgag                                                 <  1:590840/81‑3 (MQ=255)                      aaaTGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCg                                               >  2:590885/1‑81 (MQ=255)                        aTGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGtt                                             <  2:590842/81‑1 (MQ=255)                           ccTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTg                                           <  1:590843/80‑1 (MQ=255)                           ccTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGc                                          <  1:590844/81‑1 (MQ=255)                                  gATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAca                                   >  2:590883/1‑81 (MQ=255)                                    tGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatc                                 <  1:590845/81‑1 (MQ=255)                                     gggCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatca                                >  1:590887/1‑81 (MQ=255)                                      ggCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatcat                               <  2:590846/81‑1 (MQ=255)                                       gCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAAcatcat                               >  2:590886/1‑80 (MQ=255)                                       gCCAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATg                              <  2:590847/81‑1 (MQ=255)                                         cAGATGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGaa                            <  1:590848/81‑1 (MQ=255)                                             tGGTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCa                        <  1:590849/81‑1 (MQ=255)                                              ggTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGACAACATCATGAAAGc                         >  1:590850/1‑79 (MQ=255)                                               gTCGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGACAACATCATGAAAGCa                        <  2:590850/79‑1 (MQ=255)                                                 cGATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  2:590895/1‑81 (MQ=255)                                                  gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  2:590851/1‑80 (MQ=255)                                                  gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  2:590891/1‑80 (MQ=255)                                                  gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  1:590898/1‑80 (MQ=255)                                                  gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  1:590897/1‑80 (MQ=255)                                                  gATGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCt                    >  1:590893/1‑80 (MQ=255)                                                   aTGCCGATACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGa                  <  2:590852/81‑1 (MQ=255)                                                         aTACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTgg            <  2:590853/81‑1 (MQ=255)                                                          tACCGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGc           <  1:590854/81‑1 (MQ=255)                                                             cGCCTATATAACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTg         >  2:590892/1‑80 (MQ=255)                                                                   tataACCAGTCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAAtggct  <  1:590855/81‑1 (MQ=255)                                                                            |                                                                        TTGCTGGGAGCAGCTACGGGAAAATGCCTATCTGATGGGCCAGATGGTCGATGCCGATACCGCCTATATAACCAGCCGTGGCCTGCGCACATTAGGTGTGCGTTTGCGTCAACATCATGAAAGCAGTCTGAAAGTGGCTGAATGGCT  >  NC_000913/3152919‑3153065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑