Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,381	G→A	V226M (GTG→ATG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,381	0	G	A	100.0%	123.9 / NA	34	V226M (GTG→ATG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

GAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGC  >  NC_000913/353305‑353456                                                                             |                                                                            gAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAGCACATCGGCGCGCGGGTACCGatggc                                                                         >  2:75656/1‑81 (MQ=255)  aaaaTGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGatggcg                                                                        <  1:75628/81‑1 (MQ=255)     aTGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGatggcgtgg                                                                     >  1:75688/1‑81 (MQ=255)       ggCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCt                                                                   >  1:75685/1‑81 (MQ=255)        gcgcgcGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTg                                                                  >  2:75668/1‑81 (MQ=255)         cgcgcgTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTgg                                                                 <  1:75629/81‑1 (MQ=255)               tCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATcc                                                            >  1:75686/1‑80 (MQ=255)                cAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCaa                                                          >  1:75678/1‑81 (MQ=255)                 aGCGGGCGGGATGTCGATTTCGCGTCGTTTCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAAc                                                         <  1:75630/81‑1 (MQ=255)                 aGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCaa                                                          <  1:75631/80‑1 (MQ=255)                       cgggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAcc                                                   >  1:75699/1‑81 (MQ=255)                        gggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAcct                                                  >  1:75666/1‑81 (MQ=255)                        gggATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAAcct                                                  >  2:75672/1‑81 (MQ=255)                              tCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGc                                             <  1:75632/80‑1 (MQ=255)                                gATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCAtt                                          >  2:75693/1‑81 (MQ=255)                                     cgcgTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTctct                                      <  1:75633/80‑1 (MQ=255)                                        gtcgtTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTacac                                  >  1:75689/1‑81 (MQ=255)                                           gtTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTc                               >  1:75698/1‑81 (MQ=255)                                           gtTGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTc                               >  1:75687/1‑81 (MQ=255)                                            ttGCGCCATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTcc                              >  1:75697/1‑81 (MQ=255)                                                  cATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCAc                         >  2:75675/1‑80 (MQ=255)                                                  cATCAACACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACg                        <  1:75634/81‑1 (MQ=255)                                                      aaCACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGAcc                     <  1:75635/80‑1 (MQ=255)                                                      aaCACATCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCg                    <  1:607280/81‑1 (MQ=255)                                                        cacaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGc                  <  2:75636/81‑1 (MQ=255)                                                         acaTCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCa                 <  2:75637/81‑1 (MQ=255)                                                            tCGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAAc              >  2:75706/1‑81 (MQ=255)                                                             cGGCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAAcc             >  2:75708/1‑81 (MQ=255)                                                              ggCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAAcc             >  1:75705/1‑80 (MQ=255)                                                              ggCGCGCGGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCt            <  2:75638/81‑1 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTaaa         <  2:75639/81‑1 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTa           >  1:75700/1‑79 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTa           >  1:75696/1‑79 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTa           >  2:75694/1‑79 (MQ=255)                                                                 gcgcgGGTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTa           >  1:75680/1‑79 (MQ=255)                                                                      ggTACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGt    <  1:75640/81‑1 (MQ=255)                                                                        tACCGATGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGc  <  2:75641/81‑1 (MQ=255)                                                                             |                                                                            GAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGC  >  NC_000913/353305‑353456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑