Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,749	G→A	L181L (CTG→CTA)	ygiM →	putative signal transduction protein (SH3 domain)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,749	0	G	A	100.0%	96.1 / NA	27	L181L (CTG→CTA)	ygiM	putative signal transduction protein (SH3 domain)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

CCGCCAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACT  >  NC_000913/3201676‑3201825                                                                          |                                                                             ccgccAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGggcttg                                                                       <  1:601517/81‑1 (MQ=255)   gccAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGggcttggc                                                                     >  1:601567/1‑81 (MQ=255)     cAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGggcttggctt                                                                   <  2:601518/81‑1 (MQ=255)     cAGCGTACAGCTGGATGACAAACAGCGCACCAACATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGggcttggctt                                                                   >  2:601559/1‑81 (MQ=255)        cGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTtgct                                                                <  1:601519/81‑1 (MQ=255)        cGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTtgct                                                                >  2:601570/1‑81 (MQ=255)          tACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTtgctgc                                                              >  1:601565/1‑81 (MQ=255)                  gATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTc                                                        <  1:601520/79‑1 (MQ=255)                  gATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCt                                                       <  1:601521/80‑1 (MQ=255)                    tGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGt                                                    <  1:601522/81‑1 (MQ=255)                      aCAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTa                                                   <  2:601523/80‑1 (MQ=255)                        aaaCAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTg                                                >  1:601556/1‑81 (MQ=255)                         aaCAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGc                                               <  2:601524/81‑1 (MQ=255)                           cAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCg                                             <  2:601525/81‑1 (MQ=255)                            aGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCg                                             >  1:601574/1‑80 (MQ=255)                                 aCCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCt                                        >  2:601573/1‑80 (MQ=255)                                       atcatGCAATGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATccc                                  <  2:601526/80‑1 (MQ=255)                                               aTGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgca                          >  1:601566/1‑80 (MQ=255)                                                tGGTTTATGTATGGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgcaaa                        <  1:601527/81‑1 (MQ=255)                                                      atgtatgGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgcaaacgca                    >  2:601563/1‑79 (MQ=255)                                                         tatgGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGAt               <  1:601528/81‑1 (MQ=255)                                                          atgGCGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCcgcaaacgca                    >  2:601561/1‑75 (MQ=255)                                                              cGGTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTg          >  1:601562/1‑81 (MQ=255)                                                               ggTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTgg         <  2:601529/81‑1 (MQ=255)                                                                gTGGCGTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGa        >  1:601572/1‑81 (MQ=255)                                                                     gTGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAAc   >  1:601586/1‑81 (MQ=255)                                                                      tGCTAGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACt  <  2:601530/81‑1 (MQ=255)                                                                          |                                                                             CCGCCAGCGTACAGCTGGATGACAAACAGCGCACCATCATCATGCAATGGTTTATGTATGGCGGTGGCGTGCTGGGGCTTGGCTTGCTGCTCGGTCTGGTACTGCCGCACCTGATCCCAAGCCGCAAACGCAAAGATCGCTGGATGAACT  >  NC_000913/3201676‑3201825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑