Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,566	C→T	S978S (AGC→AGT)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,566	0	C	T	100.0%	112.4 / NA	31	S978S (AGC→AGT)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/15);  total (16/15)

GAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGA  >  NC_000913/3225489‑3225643                                                                              |                                                                              gAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAg                                                                            >  1:607236/1‑81 (MQ=255)       cAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCt                                                                       <  2:607190/80‑1 (MQ=255)        aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg                                                                     <  1:607191/81‑1 (MQ=255)           gcAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACt                                                                  >  1:607245/1‑81 (MQ=255)             aGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTcc                                                                <  1:607192/81‑1 (MQ=255)               tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg                                                              <  2:607193/81‑1 (MQ=255)                      aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc                                                       >  2:607249/1‑81 (MQ=255)                      aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc                                                       <  1:607194/81‑1 (MQ=255)                      aTCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTc                                                       >  2:607237/1‑81 (MQ=255)                        cACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTg                                                     >  1:607232/1‑81 (MQ=255)                               aaCCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCg                                               <  2:607195/80‑1 (MQ=255)                                aCCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGt                                              >  1:607246/1‑80 (MQ=255)                                    cGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt                                         >  1:607254/1‑81 (MQ=255)                                    cGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt                                         >  2:607239/1‑81 (MQ=255)                                     gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc                                        <  1:607196/81‑1 (MQ=255)                                      aTCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACtt                                         >  1:607251/1‑79 (MQ=255)                                      aTCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCa                                       <  1:607197/81‑1 (MQ=255)                                        cACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGc                                     >  1:607240/1‑81 (MQ=255)                                         aCCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCt                                    <  1:607198/81‑1 (MQ=255)                                            aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg                                 >  1:607247/1‑81 (MQ=255)                                            aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg                                 >  2:607244/1‑81 (MQ=255)                                                 ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCtt                             <  1:607199/80‑1 (MQ=255)                                                  ttGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTa                           >  1:607250/1‑81 (MQ=255)                                                  ttGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTa                           >  2:607235/1‑81 (MQ=255)                                                           gCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctgc                  <  1:607200/81‑1 (MQ=255)                                                            cTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGcc                 >  1:607228/1‑81 (MQ=255)                                                               cAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCgg               <  1:607201/80‑1 (MQ=255)                                                                    ccTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTg         >  1:607252/1‑81 (MQ=255)                                                                        gggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCgg     <  1:607202/81‑1 (MQ=255)                                                                          gCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGgag   <  2:607203/81‑1 (MQ=255)                                                                           cAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGgaga  <  1:607204/81‑1 (MQ=255)                                                                              |                                                                              GAGCTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGGAGA  >  NC_000913/3225489‑3225643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑