Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,290,288	A→G	Y492C (TAT→TGT)	yraJ →	putative fimbrial usher protein YraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,290,288	0	A	G	100.0%	72.3 / NA	21	Y492C (TAT→TGT)	yraJ	putative fimbrial usher protein YraJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/13);  total (8/13)

TCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCG  >  NC_000913/3290213‑3290365                                                                            |                                                                              tCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACa                                                                          <  1:621004/81‑1 (MQ=255)   gCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACAGt                                                                        <  1:621005/81‑1 (MQ=255)    cTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACAGTc                                                                       <  2:621006/81‑1 (MQ=255)                           aaCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGTAATTGGGACTGTAACAGTCGCCAAAAAAGTGAaattcaatt                                                 >  2:621024/1‑80 (MQ=255)                           aaCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAaattcaatt                                                 >  2:621032/1‑80 (MQ=255)                            aCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAattcaattca                                               <  1:621007/81‑1 (MQ=255)                                     aTTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGc                                       >  2:621038/1‑80 (MQ=255)                                            ttttAATGAAGCCAATACCCGCAATTGAGACTGTAACAGACGCCAAAAAATTGAAATTCAATTCAACATCAGCCAGACAat                               <  2:621008/81‑1 (MQ=255)                                                aaTGAAGCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATAtt                            <  2:621009/80‑1 (MQ=255)                                                 aTGAAGCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTg                          <  1:621010/81‑1 (MQ=255)                                                      gCCAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATggg                     >  1:621045/1‑81 (MQ=255)                                                        cAATACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGt                   <  1:621011/81‑1 (MQ=255)                                                         aaTACCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTa                  <  2:621012/81‑1 (MQ=255)                                                            aCCCGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGt               >  2:621056/1‑81 (MQ=255)                                                             cccGCAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTc              <  1:621013/81‑1 (MQ=255)                                                                 cAATTGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTa          <  1:621014/81‑1 (MQ=255)                                                                    ttGGGACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGc       >  2:621043/1‑81 (MQ=255)                                                                       ggACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCt     <  1:621015/80‑1 (MQ=255)                                                                        gACTGTAACCGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTcc   >  1:621046/1‑81 (MQ=255)                                                                        gACTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTcc   >  1:621041/1‑81 (MQ=255)                                                                         aCTGTAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCg  <  2:621016/81‑1 (MQ=255)                                                                            |                                                                              TCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCG  >  NC_000913/3290213‑3290365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑