Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,210	C→A	V176L (GTG→TTG)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,210	0	C	A	100.0%	102.7 / NA	29	V176L (GTG→TTG)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/9);  total (20/9)

GCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTT  >  NC_000913/3332132‑3332280                                                                               |                                                                       gcgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagc                                                                      >  2:630605/1‑81 (MQ=255)  cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc                                                                     <  1:630567/81‑1 (MQ=255)  cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc                                                                     <  2:630568/81‑1 (MQ=255)   gcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcca                                                                    >  2:630596/1‑81 (MQ=255)     aaTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATa                                                                  <  2:630569/81‑1 (MQ=255)      aTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATaa                                                                 >  1:630598/1‑81 (MQ=255)      aTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATaa                                                                 >  1:630622/1‑81 (MQ=255)                aaGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCaaa                                                       >  1:630599/1‑81 (MQ=255)                 aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc                                                      >  1:630604/1‑81 (MQ=255)                  gtgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAcc                                                     <  1:630570/81‑1 (MQ=255)                  gtgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAcc                                                     >  1:630613/1‑81 (MQ=255)                     tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc                                                  >  1:630610/1‑81 (MQ=255)                     tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc                                                  >  2:630606/1‑81 (MQ=255)                      aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg                                                 >  1:630615/1‑81 (MQ=255)                       cAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg                                                 <  2:630571/80‑1 (MQ=255)                            aaaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCa                                            <  2:630572/80‑1 (MQ=255)                             aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCAcc                                          <  2:630573/81‑1 (MQ=255)                              aaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCg                                         <  2:630574/81‑1 (MQ=255)                                cAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAc                                       >  2:630601/1‑81 (MQ=255)                                cAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAc                                       <  1:630575/81‑1 (MQ=255)                                   gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc                                    <  1:630576/81‑1 (MQ=255)                                   gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAccc                                     >  2:630618/1‑80 (MQ=255)                                      cTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCa                                   >  1:630608/1‑79 (MQ=255)                                        gCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCa                                   >  2:630612/1‑77 (MQ=255)                                           gTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATaa                            >  2:630602/1‑81 (MQ=255)                                             gAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACt                          >  2:630623/1‑81 (MQ=255)                                                     ccGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgt                  >  2:630609/1‑81 (MQ=255)                                                      cGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg                 >  2:630607/1‑81 (MQ=255)                                                      cGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg                 >  1:630617/1‑81 (MQ=255)                                                        aCGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtgt                >  1:630614/1‑80 (MQ=255)                                                              tAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGt         >  2:630619/1‑81 (MQ=255)                                                                  aCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGc     <  1:630577/81‑1 (MQ=255)                                                                     ttttGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCttt  >  2:630620/1‑81 (MQ=255)                                                                               |                                                                       GCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTT  >  NC_000913/3332132‑3332280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑