Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,665	T→G	L280R (CTG→CGG)	yhcG →	DUF1016 domain‑containing protein YhcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,665	0	T	G	100.0%	89.1 / NA	25	L280R (CTG→CGG)	yhcG	DUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/16);  total (9/16)

GTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGC  >  NC_000913/3368587‑3368740                                                                               |                                                                            gTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgccggc                                                                           >  2:638891/1‑81 (MQ=255) gTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGctgccggc                                                                           >  1:638895/1‑81 (MQ=255)  tCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCt                                                                          <  2:638857/81‑1 (MQ=255)    ggCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTaa                                                                        <  1:638859/81‑1 (MQ=255)    ggCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTaa                                                                        <  1:638858/81‑1 (MQ=255)     gCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAAt                                                                       <  1:638860/81‑1 (MQ=255)        gCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAAtcgt                                                                    <  1:638861/81‑1 (MQ=255)            aGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGAt                                                                >  2:638893/1‑81 (MQ=255)              gTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCt                                                              <  2:638862/81‑1 (MQ=255)               ttACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTa                                                             <  1:638863/81‑1 (MQ=255)                    cATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGt                                                        >  1:638902/1‑81 (MQ=255)                    cATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGt                                                        >  2:638904/1‑81 (MQ=255)                         atgaCAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGc                                                    <  2:638864/80‑1 (MQ=255)                           gaCAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCaaa                                                 >  1:638898/1‑81 (MQ=255)                            aCAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAAt                                                >  1:638906/1‑81 (MQ=255)                             cAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAAtt                                               <  1:638866/81‑1 (MQ=255)                             cAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAAtt                                               <  1:638865/81‑1 (MQ=255)                              aaCTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAAtt                                               >  1:638899/1‑80 (MQ=255)                                         tgtCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGa                                   <  1:638867/81‑1 (MQ=255)                                           tCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGAt                                  <  1:638868/80‑1 (MQ=255)                                             gATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGc                                <  2:638869/80‑1 (MQ=255)                                                  gctgTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGa                            >  2:638907/1‑79 (MQ=255)                                                   ctgTTTTTCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACa                          <  2:638870/80‑1 (MQ=255)                                                      tttttCCACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGa                        <  1:638871/79‑1 (MQ=255)                                                           ccACCGCCGTTTACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAata                  <  1:638872/80‑1 (MQ=255)                                                                      tACGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAAc       >  2:638913/1‑80 (MQ=255)                                                                       aCGCTGCCGGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTa     >  2:638873/1‑81 (MQ=255)                                                                           tgccggcTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGc  >  1:638905/1‑80 (MQ=255)                                                                               |                                                                            GTCGGCAGCGAAGGTTACGCATTGATGACAACTGGTTTCGTGTCGATCTGCTGTTTTTCCACCGCCGTTTACGCTGCCTGCTAATCGTCGATCTAAAAGTGGGCAAATTCAGCTATAGCGATGCCGGACAGATGAATATGTATCTCAACTACGC  >  NC_000913/3368587‑3368740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑