Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,944	G→A	V26M (GTG→ATG)	dcuD →	putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,944	0	G	A	100.0%	51.7 / NA	15	V26M (GTG→ATG)	dcuD	putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

ATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTC  >  NC_000913/3374869‑3375012                                                                            |                                                                     aTGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGatggtg                                                                 >  1:640350/1‑81 (MQ=255) aTGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGatggtg                                                                 <  2:640317/81‑1 (MQ=255)       ggCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTgctggct                                                           >  2:640340/1‑81 (MQ=255)          aTAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGcc                                                        >  2:640352/1‑81 (MQ=255)                 tatCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTa                                                 >  1:640361/1‑81 (MQ=255)                       tCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTcc                                           <  1:640319/81‑1 (MQ=255)                       tCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTcc                                           <  1:640318/81‑1 (MQ=255)                                   ttaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCg                               >  2:640351/1‑81 (MQ=255)                                        aTGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTc                          >  1:640355/1‑81 (MQ=255)                                         tGGGCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCt                         <  1:640320/81‑1 (MQ=255)                                          gggCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTg                        >  1:640356/1‑81 (MQ=255)                                            gCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGt                      >  2:640366/1‑81 (MQ=255)                                            gCTATTTGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGt                      <  2:640321/81‑1 (MQ=255)                                                tttGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAgg                  <  1:640322/81‑1 (MQ=255)                                                 ttGATCCTGAAAAACTACAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAggg                 >  1:640357/1‑81 (MQ=255)                                                                 aCAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTc  >  2:640371/1‑80 (MQ=255)                                                                 aCAAACCTCAGATGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGCCTGGTTAGGGTTCGGTGGTGTACTc  >  1:640353/1‑80 (MQ=255)                                                                            |                                                                     ATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTC  >  NC_000913/3374869‑3375012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑