Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,397,596	A→T	F67Y (TTT→TAT)	rng ←	RNase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,397,596	0	A	T	100.0%	127.3 / NA	35	F67Y (TTT→TAT)	rng	RNase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/20);  total (15/20)

GTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGT  >  NC_000913/3397520‑3397671                                                                             |                                                                            gTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgc                                                                         >  1:645614/1‑81 (MQ=255)  tCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgcg                                                                        <  1:645566/81‑1 (MQ=255)  tCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgc                                                                         <  2:645565/80‑1 (MQ=255)   cgcgCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCgg                                                                       >  1:645618/1‑81 (MQ=255)      gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCtt                                                                    <  1:645567/81‑1 (MQ=255)      gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCtt                                                                    >  2:645625/1‑81 (MQ=255)          cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATc                                                                <  1:645568/81‑1 (MQ=255)          cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATc                                                                >  1:645615/1‑81 (MQ=255)               aTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGcc                                                           >  1:645621/1‑81 (MQ=255)                      ttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAata                                                      <  2:645569/79‑1 (MQ=255)                         ttcttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTa                                                  >  2:645633/1‑80 (MQ=255)                         ttcttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTAc                                                 <  1:645570/81‑1 (MQ=255)                              cACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACaa                                               <  1:645571/78‑1 (MQ=255)                               aCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAg                                            <  1:645572/80‑1 (MQ=255)                                     cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTg                                     >  1:645603/1‑81 (MQ=255)                                     cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTg                                     >  1:645629/1‑81 (MQ=255)                                       cacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCa                                   <  2:645573/81‑1 (MQ=255)                                        acaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAGGCCGCCTGCAt                                  <  1:645574/81‑1 (MQ=255)                                         caTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATa                                 <  1:645575/81‑1 (MQ=255)                                          aTTCGGTGTGCGGCATGATGTCGGATGCATGGAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                <  2:645576/81‑1 (MQ=255)                                          aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                >  2:645619/1‑81 (MQ=255)                                          aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                <  1:645577/81‑1 (MQ=255)                                             cGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAccc                              <  2:645578/80‑1 (MQ=255)                                             cGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCg                             >  2:645616/1‑81 (MQ=255)                                               gtgtGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCgg                            >  1:645617/1‑80 (MQ=255)                                                 gtgCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAg                         >  1:645630/1‑81 (MQ=255)                                                  tgCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGt                        <  2:645579/81‑1 (MQ=255)                                                           atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACtt               <  2:645580/81‑1 (MQ=255)                                                           atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACt                >  2:645623/1‑80 (MQ=255)                                                           atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCAGCCTGCATACCCGGAAGTACACGACtt               <  2:645581/81‑1 (MQ=255)                                                              tCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTac             <  2:645582/80‑1 (MQ=255)                                                                 gATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGa         <  1:645583/81‑1 (MQ=255)                                                                 gATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGa         >  2:645634/1‑81 (MQ=255)                                                                  aTGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGAc        >  2:645620/1‑81 (MQ=255)                                                                       tGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTg   <  2:645584/81‑1 (MQ=255)                                                                         aagatACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGt  <  2:645585/80‑1 (MQ=255)                                                                             |                                                                            GTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGT  >  NC_000913/3397520‑3397671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑