Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	359,674	G→A	G62G (GGG→GGA)	cynS →	cyanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	359,674	0	G	A	100.0%	110.8 / NA	31	G62G (GGG→GGA)	cynS	cyanase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

CGGTCTGGCAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGGGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACC  >  NC_000913/359596‑359747                                                                               |                                                                          cGGTCTGGCAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGc                                                                         <  2:76998/81‑1 (MQ=255)       ggCAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCt                                                                   >  2:77036/1‑81 (MQ=255)        gCAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTg                                                                  <  1:76999/81‑1 (MQ=255)         cAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTg                                                                  >  1:77040/1‑80 (MQ=255)         cAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTg                                                                  >  1:77048/1‑80 (MQ=255)            aaGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGAtc                                                              <  1:77000/81‑1 (MQ=255)                  ttGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTcgac                                                         >  2:910361/1‑80 (MQ=255)                       aCCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGa                                                    <  2:77001/80‑1 (MQ=255)                           cgGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCa                                               >  2:77045/1‑81 (MQ=255)                            ggCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCAt                                              >  1:77043/1‑81 (MQ=255)                              cTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTc                                            <  1:77002/81‑1 (MQ=255)                              cTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTc                                            >  1:77041/1‑81 (MQ=255)                                       gTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGc                                   <  2:77003/81‑1 (MQ=255)                                          agcagGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAg                                 <  1:77004/80‑1 (MQ=255)                                            cagGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAgatg                              <  1:77005/81‑1 (MQ=255)                                            cagGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAgatg                              <  2:77006/81‑1 (MQ=255)                                              ggCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTGCTGTTGCAgatgat                            >  2:77053/1‑81 (MQ=255)                                               gcgcTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGAtt                           >  1:77046/1‑81 (MQ=255)                                                 gcTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTcc                         <  2:77007/81‑1 (MQ=255)                                                 gcTTCCTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTc                          >  2:77051/1‑80 (MQ=255)                                                      cTGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGc                    <  2:77008/81‑1 (MQ=255)                                                       tGCCGACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCg                   >  2:77055/1‑81 (MQ=255)                                                           gACGCCGCCCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGc               >  2:77052/1‑81 (MQ=255)                                                              gccgccCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTg             >  2:77054/1‑80 (MQ=255)                                                              gccgccCGCCTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGc            <  2:77009/81‑1 (MQ=255)                                                                 gcccgccTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCAtt         <  2:77010/81‑1 (MQ=255)                                                                    cgccTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATtgat      <  2:77011/81‑1 (MQ=255)                                                                    cgccTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATtgat      >  1:77047/1‑81 (MQ=255)                                                                       cTGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGAc   >  1:77044/1‑81 (MQ=255)                                                                        tGGTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGAcc  >  2:77058/1‑81 (MQ=255)                                                                          gTCGGAGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGAcc  <  2:77012/79‑1 (MQ=255)                                                                               |                                                                          CGGTCTGGCAGAAGCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTGGTCGGGGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTGCGTGGCTGCATTGATGACC  >  NC_000913/359596‑359747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑