Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,403,864	C→T	A127A (GCC→GCT)	yhdH →	acrylyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,403,864	0	C	T	100.0%	116.7 / NA	34	A127A (GCC→GCT)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

CGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTG  >  NC_000913/3403790‑3403939                                                                           |                                                                            cgAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGttt                                                                        >  2:647134/1‑80 (MQ=255)   aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTAc                                                                     >  2:647162/1‑81 (MQ=255)   aGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTAc                                                                     >  1:647142/1‑81 (MQ=255)      gAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGc                                                                  >  2:647141/1‑81 (MQ=255)         aGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCAt                                                               <  2:647091/81‑1 (MQ=255)          ggTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATg                                                              >  1:647137/1‑81 (MQ=255)           gTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGc                                                             <  1:647092/81‑1 (MQ=255)              aCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgt                                                          <  1:647093/81‑1 (MQ=255)              aCTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCtgt                                                          >  1:647165/1‑81 (MQ=255)                     ggTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGAt                                                   >  1:647147/1‑81 (MQ=255)                         gCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCGGTGTGTGATGgcg                                               >  1:647151/1‑81 (MQ=255)                          ccATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGgcg                                               <  2:647094/80‑1 (MQ=255)                             tGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTg                                            >  1:647163/1‑80 (MQ=255)                              gCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTgg                                           >  2:647149/1‑80 (MQ=255)                                   aaGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATg                                     <  1:647095/81‑1 (MQ=255)                                       gCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCgg                                 >  2:647153/1‑81 (MQ=255)                                        cTGGACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAGGATGCCGgt                                <  1:647096/81‑1 (MQ=255)                                          ggACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGgtgt                              >  2:647177/1‑81 (MQ=255)                                           gACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGgtgt                              <  2:647097/80‑1 (MQ=255)                                           gACGCGCGTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGtt                             >  1:647154/1‑81 (MQ=255)                                               cgcgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGcc                         <  1:647098/81‑1 (MQ=255)                                                 cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       >  1:647159/1‑81 (MQ=255)                                                 cgTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       <  1:647099/81‑1 (MQ=255)                                                  gTAAAGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCg                       >  2:647160/1‑80 (MQ=255)                                                    aaaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    >  1:647152/1‑81 (MQ=255)                                                     aaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    <  1:647100/80‑1 (MQ=255)                                                     aaGCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAg                    <  1:647101/80‑1 (MQ=255)                                                       gCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                 <  2:647103/81‑1 (MQ=255)                                                       gCAATGATTATCGGTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGAc                 <  2:647102/81‑1 (MQ=255)                                                                   ggTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGt      <  1:647104/80‑1 (MQ=255)                                                                   ggTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTc     >  1:647176/1‑81 (MQ=255)                                                                    gTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCg    >  1:647173/1‑81 (MQ=255)                                                                    gTACTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCg    >  2:647157/1‑81 (MQ=255)                                                                      aCTGCTGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGt   >  2:647158/1‑80 (MQ=255)                                                                       ctgctgGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTg  <  1:647105/80‑1 (MQ=255)                                                                           |                                                                            CGAGTGAAAGGTGACTGGCTGGTTGCCATGCCGCAAGGGCTGGACGCGCGTAAAGCAATGATTATCGGTACTGCCGGTTTTACCGCCATGCTGTGTGTGATGGCGCTGGAAGATGCCGGTGTTCGCCCGCAGGACGGGGAGATTGTCGTG  >  NC_000913/3403790‑3403939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑