Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,435,342	G→A	E46K (GAG→AAG)	rsmB →	16S rRNA m(5)C967 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,435,342	0	G	A	100.0%	95.8 / NA	27	E46K (GAG→AAG)	rsmB	16S rRNA m(5)C967 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

GCAAGGGCAATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCC  >  NC_000913/3435266‑3435412                                                                             |                                                                       gCAAGGGCAATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGtt                                                                    <  1:653987/81‑1 (MQ=255)        cAATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCttt                                                             >  1:654039/1‑81 (MQ=255)          aTCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTgg                                                           <  2:653988/81‑1 (MQ=255)           tCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGc                                                          <  2:653989/81‑1 (MQ=255)                 aGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTg                                                    <  1:653990/81‑1 (MQ=255)                 aGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTg                                                    >  1:654021/1‑81 (MQ=255)                     aCATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTa                                                <  1:653991/81‑1 (MQ=255)                      cATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTAc                                               <  1:653992/81‑1 (MQ=255)                         tCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCt                                            >  2:654038/1‑81 (MQ=255)                           tGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCATAAGTTGTGCTTTGGCGTACTGCGTACGCttt                                          >  2:654026/1‑81 (MQ=255)                             ccACCGCTCCAGCAAAAAGTTTCCGATAATGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTc                                         <  2:653993/80‑1 (MQ=255)                                  gCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGtt                                   <  2:653994/81‑1 (MQ=255)                                  gCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGt                                    >  2:654040/1‑80 (MQ=255)                                   cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGt                                    >  2:654022/1‑79 (MQ=255)                                   cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTa                                  <  1:653995/81‑1 (MQ=255)                                     ccAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGa                                >  1:654028/1‑81 (MQ=255)                                     ccAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGa                                >  2:654025/1‑81 (MQ=255)                                      cAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGa                                <  1:653996/80‑1 (MQ=255)                                          aaaaaGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGc                           >  2:654030/1‑81 (MQ=255)                                              aGTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGAt                       <  2:653997/81‑1 (MQ=255)                                               gTTTCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGAt                       >  2:654035/1‑80 (MQ=255)                                                 ttCCGATAAAGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATtaa                    >  2:654031/1‑81 (MQ=255)                                                          aGACAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGTTTAATAAGTTAAt           >  2:654036/1‑81 (MQ=255)                                                             cAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGc        >  1:654037/1‑81 (MQ=255)                                                             cAAAGCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGc        <  2:653998/81‑1 (MQ=255)                                                                 gCACTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGt    <  1:653999/81‑1 (MQ=255)                                                                   aCTTCTTCAAAAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTcc  <  2:654000/81‑1 (MQ=255)                                                                             |                                                                       GCAAGGGCAATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCC  >  NC_000913/3435266‑3435412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑