Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,447,485	A→G	F95L (TTC→CTC)	rplX ←	50S ribosomal subunit protein L24

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,447,485	0	A	G	100.0%	91.6 / NA	26	F95L (TTC→CTC)	rplX	50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

GTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCT  >  NC_000913/3447409‑3447558                                                                             |                                                                          gTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACg                                                                        <  2:656798/80‑1 (MQ=255)   cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGAc                                                                     >  2:656848/1‑81 (MQ=255)        tagtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACttttt                                                                >  1:656841/1‑81 (MQ=255)             aTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCg                                                           >  1:656845/1‑81 (MQ=255)                   cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCg                                                     <  2:656800/81‑1 (MQ=255)                   cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCg                                                     <  2:656799/81‑1 (MQ=255)                      tttCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAAt                                                  <  1:656801/81‑1 (MQ=255)                        tCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATc                                                 >  2:656802/1‑80 (MQ=255)                          gCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTa                                               <  2:656803/80‑1 (MQ=255)                           ccATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTa                                               >  1:656849/1‑79 (MQ=255)                                     actCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACgg                                   >  2:656836/1‑81 (MQ=255)                                      ctCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGt                                  <  2:656804/81‑1 (MQ=255)                                        ccAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGt                                  >  2:656840/1‑79 (MQ=255)                                         cAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                               >  1:656844/1‑81 (MQ=255)                                           aaTTACTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGcc                             >  2:656838/1‑81 (MQ=255)                                                cTTGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGcc                        >  1:656847/1‑81 (MQ=255)                                                 ttGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCg                       >  1:656832/1‑81 (MQ=255)                                                  tGATAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCgg                      >  2:656839/1‑81 (MQ=255)                                                    aTAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGATTCTAAAGCCTACACGGTCAGCCTTGCCGGtt                    >  2:656852/1‑81 (MQ=255)                                                    aTAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGt                     <  1:656805/80‑1 (MQ=255)                                                     tAGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTg                   <  2:656806/81‑1 (MQ=255)                                                      aGTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGc                  >  2:656843/1‑81 (MQ=255)                                                       gTTTCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGcc                 <  2:656807/81‑1 (MQ=255)                                                        tttCGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                <  1:656808/81‑1 (MQ=255)                                                           cGCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAt             >  1:656833/1‑81 (MQ=255)                                                            gCTGTTAGACTTGAAGAGACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAt             <  2:656809/80‑1 (MQ=255)                                                                        gaagagACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt  <  2:656810/79‑1 (MQ=255)                                                                             |                                                                          GTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCT  >  NC_000913/3447409‑3447558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑