Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,692	G→T	A608E (GCG→GAG)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,692	0	G	T	100.0%	118.6 / NA	33	A608E (GCG→GAG)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/17);  total (16/17)

CACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTC  >  NC_000913/3471615‑3471762                                                                              |                                                                       cACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCtt                                                                     >  1:662122/1‑81 (MQ=255)    cGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCt                                                                  >  2:662136/1‑81 (MQ=255)     gATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCtctttctt                                                                 <  1:662079/81‑1 (MQ=255)      aTAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTa                                                                <  2:662080/81‑1 (MQ=255)       tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa                                                               <  1:662081/81‑1 (MQ=255)       tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa                                                               <  2:662082/81‑1 (MQ=255)           gTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGcc                                                           >  1:662132/1‑81 (MQ=255)            tCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCct                                                          <  1:662083/81‑1 (MQ=255)                 ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCttt                                                     <  1:662084/81‑1 (MQ=255)                  gtgtTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTa                                                    <  1:662085/81‑1 (MQ=255)                    gtTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTa                                                    >  2:662143/1‑79 (MQ=255)                            ccGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATa                                           <  2:662086/80‑1 (MQ=255)                              ggAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAg                                          >  1:662146/1‑79 (MQ=255)                              ggAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAg                                          >  1:662133/1‑79 (MQ=255)                               gAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAag                                       <  2:662087/81‑1 (MQ=255)                                aGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAag                                       >  1:662138/1‑80 (MQ=255)                                      tACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAg                                >  1:662135/1‑81 (MQ=255)                                        cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGtt                              <  1:662088/81‑1 (MQ=255)                                           cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAATGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt                             <  1:662089/79‑1 (MQ=255)                                           cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGttt                             <  2:662090/79‑1 (MQ=255)                                           cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                            >  2:662134/1‑80 (MQ=255)                                             aCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAAc                         <  2:662091/81‑1 (MQ=255)                                               cTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGc                       >  1:662137/1‑81 (MQ=255)                                                  cATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGcc                      <  2:662092/79‑1 (MQ=255)                                                     gATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                  >  2:662140/1‑80 (MQ=255)                                                     gATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTc                 <  1:662093/81‑1 (MQ=255)                                                        cGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCa                >  2:662151/1‑79 (MQ=255)                                                         ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag             >  1:662145/1‑81 (MQ=255)                                                          gCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTCTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag             >  2:662142/1‑80 (MQ=255)                                                            tCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAgaggag          >  2:662147/1‑81 (MQ=255)                                                                 cAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAAc     <  2:662094/81‑1 (MQ=255)                                                                  aGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg    >  2:662144/1‑81 (MQ=255)                                                                   gAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGt   <  1:662095/81‑1 (MQ=255)                                                                    aaCTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTc  <  1:662096/81‑1 (MQ=255)                                                                              |                                                                       CACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTC  >  NC_000913/3471615‑3471762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑