Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,005	G→A	M1M (ATG→ATA) †	nirC →	nitrite transporter NirC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,005	0	G	A	100.0%	132.2 / NA	36	M1M (ATG→ATA) †	nirC	nitrite transporter NirC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

CTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCT  >  NC_000913/3496930‑3497081                                                                            |                                                                             cTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTa                                                                          >  1:668089/1‑80 (MQ=255)   tCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTa                                                                          >  2:668087/1‑78 (MQ=255)    cGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACa                                                                        <  2:668057/79‑1 (MQ=255)    cGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACa                                                                        <  1:668058/79‑1 (MQ=255)       tataTTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGacac                                                                   <  2:668059/81‑1 (MQ=255)        atatTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCATATATTTACAGacac                                                                   >  2:668093/1‑80 (MQ=255)        atatTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGacac                                                                   >  1:668097/1‑80 (MQ=255)         tatTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACt                                                                  <  1:668060/80‑1 (MQ=255)          atTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTAt                                                                >  2:668084/1‑81 (MQ=255)              cAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATtaa                                                             >  1:668091/1‑80 (MQ=255)                ggACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATtaataa                                                          <  1:668061/81‑1 (MQ=255)                   cATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAgtg                                                       <  1:668062/81‑1 (MQ=255)                    aTTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAgtgt                                                      >  2:668086/1‑81 (MQ=255)                         ttAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCgg                                                  <  1:668064/80‑1 (MQ=255)                         ttAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCgg                                                  <  1:668063/80‑1 (MQ=255)                          tAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGc                                                 <  1:668065/80‑1 (MQ=255)                            aaCTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTa                                               <  2:668066/80‑1 (MQ=255)                              cTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACg                                            >  1:668098/1‑81 (MQ=255)                                 tttGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCt                                          <  1:668067/80‑1 (MQ=255)                                 tttGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTg                                         >  1:668096/1‑81 (MQ=255)                                  ttGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTgc                                        >  1:668117/1‑81 (MQ=255)                                       ttATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCa                                   >  1:668092/1‑81 (MQ=255)                                       ttATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCa                                   >  1:668101/1‑81 (MQ=255)                                         aTTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCAtt                                 >  1:668103/1‑81 (MQ=255)                                          ttttttGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCAtt                                 <  2:668068/80‑1 (MQ=255)                                              ttGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACg                            >  2:668123/1‑81 (MQ=255)                                                 tttttATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCt                         <  2:668069/81‑1 (MQ=255)                                                 tttttATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCt                         >  1:668088/1‑81 (MQ=255)                                                  ttttATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTg                        >  2:668090/1‑81 (MQ=255)                                                       ttttttAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGc                   <  2:668070/81‑1 (MQ=255)                                                           ttAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCaaa                >  2:668094/1‑80 (MQ=255)                                                               aGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAAcc           >  2:668115/1‑81 (MQ=255)                                                                ggATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAAccc          <  1:668071/81‑1 (MQ=255)                                                                   tAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCt       >  1:668131/1‑81 (MQ=255)                                                                   tAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCt       >  1:668095/1‑81 (MQ=255)                                                                        aaaTATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCt  >  1:668107/1‑81 (MQ=255)                                                                            |                                                                             CTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCT  >  NC_000913/3496930‑3497081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑