Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,499,007	C→T	L394L (CTG→TTG)	cysG →	siroheme synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,499,007	0	C	T	100.0%	113.0 / NA	32	L394L (CTG→TTG)	cysG	siroheme synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

GGAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGA  >  NC_000913/3498931‑3499082                                                                             |                                                                            ggAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTg                                                                           <  2:668527/79‑1 (MQ=255) ggAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGAt                                                                         <  1:668526/81‑1 (MQ=255)     aaCCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGaa                                                                     >  2:668562/1‑81 (MQ=255)         tGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACg                                                                 >  1:668573/1‑81 (MQ=255)          ggcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACg                                                                 >  2:668571/1‑80 (MQ=255)           gcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACgg                                                                <  1:668528/80‑1 (MQ=255)           gcggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGa                                                               <  2:668529/81‑1 (MQ=255)            cggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGaa                                                              >  1:668567/1‑81 (MQ=255)             ggcAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAAt                                                             <  1:668530/81‑1 (MQ=255)                 gAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCa                                                         <  1:668531/81‑1 (MQ=255)                     aaCAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGc                                                      >  2:668563/1‑80 (MQ=255)                        aGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGaaa                                                  <  1:668532/81‑1 (MQ=255)                         gACGCTGGTGTTCTATATGGGGTTGAATCAGACCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAAt                                                 <  2:668533/81‑1 (MQ=255)                            gCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGcc                                              >  1:668566/1‑81 (MQ=255)                               ggTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGt                                           >  2:668568/1‑81 (MQ=255)                                   ttCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGc                                        >  1:668570/1‑80 (MQ=255)                                   ttCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCa                                       <  1:668534/81‑1 (MQ=255)                                        tatGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAAtt                                    <  1:668536/79‑1 (MQ=255)                                        tatGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAAt                                     <  1:668535/78‑1 (MQ=255)                                          tGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTc                                 <  2:668537/80‑1 (MQ=255)                                            gggTTGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGa                               >  2:668581/1‑80 (MQ=255)                                               ttGAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAAc                           <  2:668538/81‑1 (MQ=255)                                                 gAATCAGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACgg                         >  2:668574/1‑81 (MQ=255)                                                      aGGCCGCGACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACg                     <  1:668539/80‑1 (MQ=255)                                                             gACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCAc             <  2:668540/81‑1 (MQ=255)                                                             gACTATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCAc             >  2:668585/1‑81 (MQ=255)                                                                tATTCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACg            >  1:668586/1‑79 (MQ=255)                                                                  ttCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCa          <  2:668541/79‑1 (MQ=255)                                                                  ttCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAg         <  2:668542/80‑1 (MQ=255)                                                                   tCAGCAAAAGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAgcg       >  2:668584/1‑81 (MQ=255)                                                                     aggaaaaGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAgcgc      <  2:668543/77‑1 (MQ=255)                                                                        aaaaGTTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGa  <  1:668544/81‑1 (MQ=255)                                                                             |                                                                            GGAAAACCTGGCGGCAGAAAAACAGACGCTGGTGTTCTATATGGGGTTGAATCAGGCCGCGACTATTCAGCAAAAGCTGATTGAACACGGAATGCCAGGCGAAATGCCGGTGGCAATTGTCGAAAACGGTACGGCAGTCACGCAGCGCGTGA  >  NC_000913/3498931‑3499082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑