Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,589,355	C→T	V225M (GTG→ATG)	ugpE ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,589,355	0	C	T	100.0%	81.6 / NA	23	V225M (GTG→ATG)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

TGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCC  >  NC_000913/3589281‑3589428                                                                           |                                                                          tGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCgg                                                                      >  1:690311/1‑80 (MQ=255)   aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGta                                                                    <  1:690266/80‑1 (MQ=255)   aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGt                                                                     <  1:690267/79‑1 (MQ=255)   aGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGt                                                                     <  1:690268/79‑1 (MQ=255)    gTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGtaat                                                                  <  2:690269/81‑1 (MQ=255)        cATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTaataat                                                               >  2:690314/1‑80 (MQ=255)        cATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTaataat                                                               >  1:690324/1‑80 (MQ=255)          ttCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATcaa                                                            >  1:690317/1‑81 (MQ=255)               tggtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACg                                                       <  1:690270/81‑1 (MQ=255)                 gtgCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACgg                                                      <  2:690271/80‑1 (MQ=255)                    ccTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCcac                                                  <  1:690272/81‑1 (MQ=255)                        cGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACaaa                                               <  1:690273/80‑1 (MQ=255)                            tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACt                                           >  2:690316/1‑80 (MQ=255)                                 cGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTc                                      >  1:690313/1‑80 (MQ=255)                                          cTTTGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGa                            <  2:690274/81‑1 (MQ=255)                                            ttGATCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGAt                           >  2:690320/1‑80 (MQ=255)                                               aTCCCTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAg                       <  1:690275/81‑1 (MQ=255)                                                   cTGCCACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGa                   >  2:690331/1‑81 (MQ=255)                                                      ccACGGTGGTGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGAt                  <  1:690276/79‑1 (MQ=255)                                                          ggtggtGCCGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACaaa            >  2:690323/1‑81 (MQ=255)                                                                 ccGAGATCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGcgcc     <  1:690277/81‑1 (MQ=255)                                                                   gagaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGcgccgc   >  1:690340/1‑81 (MQ=255)                                                                    agaTCCATATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCgccgcc  <  1:690278/81‑1 (MQ=255)                                                                           |                                                                          TGAGTTCCATTCCGTGGTGCCTTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCC  >  NC_000913/3589281‑3589428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑