Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,607,423	G→T	A325S (GCC→TCC)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,607,423	0	G	T	100.0%	191.5 / NA	52	A325S (GCC→TCC)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/30);  total (22/30)

GATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTC  >  NC_000913/3607348‑3607500                                                                            |                                                                              gATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCAtt                                                                          <  2:694697/81‑1 (MQ=255)    aaaGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGAc                                                                       >  1:694774/1‑81 (MQ=255)      aGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCg                                                                     <  1:694698/81‑1 (MQ=255)      aGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCg                                                                     >  1:694751/1‑81 (MQ=255)      aGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCg                                                                     >  2:694759/1‑81 (MQ=255)       gTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCgg                                                                    <  2:694699/81‑1 (MQ=255)              ctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCt                                                               <  2:694700/79‑1 (MQ=255)              ctgGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCt                                                               <  2:694701/79‑1 (MQ=255)                 gTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAAc                                                          <  1:694702/81‑1 (MQ=255)                    ccaccaGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGa                                                       <  1:694703/81‑1 (MQ=255)                     caccaGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGAt                                                      >  1:694764/1‑81 (MQ=255)                      accaGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATc                                                     <  2:694705/81‑1 (MQ=255)                      accaGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATc                                                     <  1:694704/81‑1 (MQ=255)                       ccaGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCg                                                    <  2:694706/81‑1 (MQ=255)                        caGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCga                                                   <  1:694707/81‑1 (MQ=255)                          gCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCgaag                                                 <  1:694708/81‑1 (MQ=255)                          gCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCg                                                    >  1:694775/1‑78 (MQ=255)                            gTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCgaagaa                                               <  2:694709/81‑1 (MQ=255)                             tAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGaagaag                                              >  2:694755/1‑81 (MQ=255)                               gACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGcc                                            <  1:694710/81‑1 (MQ=255)                               gACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGcc                                            >  1:694758/1‑81 (MQ=255)                                aCCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGcc                                            >  1:694778/1‑80 (MQ=255)                                aCCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGcc                                            >  1:694784/1‑80 (MQ=255)                                 ccGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGa                                          <  2:694711/81‑1 (MQ=255)                                    tCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGaa                                         >  1:694768/1‑79 (MQ=255)                                     cTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAagag                                      >  1:694763/1‑81 (MQ=255)                                        gTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCg                                    <  1:694712/80‑1 (MQ=255)                                        gTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCg                                    <  2:694714/80‑1 (MQ=255)                                        gTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGt                                   <  1:694713/81‑1 (MQ=255)                                         tGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTc                                  <  1:694715/81‑1 (MQ=255)                                           aCGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgc                                <  2:694717/81‑1 (MQ=255)                                           aCGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTcgc                                <  1:694716/81‑1 (MQ=255)                                             gTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCgcgc                              >  1:694757/1‑81 (MQ=255)                                             gTTGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCgcgc                              >  1:694762/1‑81 (MQ=255)                                               tGGAAGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTc                            >  1:694767/1‑81 (MQ=255)                                                   aGTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAt                        <  2:694718/81‑1 (MQ=255)                                                    gTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAtt                       >  1:694769/1‑81 (MQ=255)                                                    gTGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCAtt                       <  2:694719/81‑1 (MQ=255)                                                     tGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTg                      <  1:694720/81‑1 (MQ=255)                                                     tGCTGTCAGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTg                      <  2:694721/81‑1 (MQ=255)                                                            aGAACCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGtt               >  2:694780/1‑81 (MQ=255)                                                              aaCCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTa             >  1:694788/1‑81 (MQ=255)                                                              aaCCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTa             >  2:694765/1‑81 (MQ=255)                                                               aCCGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTAt            >  2:694766/1‑81 (MQ=255)                                                                ccGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATc           <  2:694722/81‑1 (MQ=255)                                                                ccGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATc           <  2:694723/81‑1 (MQ=255)                                                                ccGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATc           >  1:694779/1‑81 (MQ=255)                                                                 cGGGAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCg          <  2:694724/81‑1 (MQ=255)                                                                   ggAGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGAc        >  2:694770/1‑81 (MQ=255)                                                                     aGCCAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCg      <  2:694726/81‑1 (MQ=255)                                                                        cAGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGttt   >  2:694783/1‑81 (MQ=255)                                                                         aGCTCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTc  <  1:694727/81‑1 (MQ=255)                                                                            |                                                                              GATAAAGTCCCTGCTGGTGCCACCAGCGTAGACCGTCTGGTGACGTTGGAAGTGCTGTCAGAACCGGGAGCCAGCGCCATTGACCGGATTCTGAAACTGATCGAAGAAGCCGAAGAGCGTCGCGCTCCCATTGAGCGGTTTATCGACCGTTTC  >  NC_000913/3607348‑3607500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑