Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,608,490	C→T	A680A (GCC→GCT)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,608,490	0	C	T	100.0%	139.9 / NA	39	A680A (GCC→GCT)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/22);  total (17/22)

AACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATG  >  NC_000913/3608412‑3608562                                                                               |                                                                         aaCCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTaa                                                                        >  2:872830/1‑81 (MQ=255) aaCCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTaa                                                                        >  1:695071/1‑81 (MQ=255)   ccgccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAata                                                                      <  1:695020/81‑1 (MQ=255)     gccgACGCAGCACTAACCCATAACCACCTGCGCGGCCTGGTTCAAATGATTGAACTGGCACGCGCCACTCACGCTAATTTc                                                                    >  2:695073/1‑81 (MQ=255)      ccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATcc                                                                   >  2:695079/1‑81 (MQ=255)        gACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc                                                                 >  2:695081/1‑81 (MQ=255)         aCGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc                                                                 >  2:695064/1‑80 (MQ=255)           gcagcaTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAg                                                              <  2:695021/81‑1 (MQ=255)               caTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACa                                                          >  2:695075/1‑81 (MQ=255)                aTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACAt                                                         >  1:695078/1‑81 (MQ=255)                 ttAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATc                                                        <  1:695022/81‑1 (MQ=255)                 ttAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATc                                                        <  1:695023/81‑1 (MQ=255)                    aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACt                                                     <  1:695025/81‑1 (MQ=255)                    aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACt                                                     <  2:695024/81‑1 (MQ=255)                     cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTa                                                    >  1:695076/1‑81 (MQ=255)                       cATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTAtt                                                  <  1:695026/81‑1 (MQ=255)                              accTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTgg                                           <  2:695027/81‑1 (MQ=255)                                cTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTgggg                                         >  1:695080/1‑81 (MQ=255)                                 tGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGc                                        >  1:695089/1‑81 (MQ=255)                                      ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGaaa                                   <  2:695028/81‑1 (MQ=255)                                      ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                     >  1:695088/1‑79 (MQ=255)                                      ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                     >  1:695084/1‑79 (MQ=255)                                        ccTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg                                 <  1:695029/81‑1 (MQ=255)                                              gCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCtt                           >  2:695030/1‑81 (MQ=255)                                               cAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCtt                           <  1:695031/80‑1 (MQ=255)                                               cAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTc                          <  2:695032/81‑1 (MQ=255)                                               cAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTc                          <  2:695033/81‑1 (MQ=255)                                                aaaTGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTcc                         <  2:695034/81‑1 (MQ=255)                                                 aaTGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCt                        <  1:695035/81‑1 (MQ=255)                                                  aTGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTc                       <  1:695036/81‑1 (MQ=255)                                                    gATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt                     >  1:695083/1‑81 (MQ=255)                                                      ttGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTca                   <  2:695037/81‑1 (MQ=255)                                                         aaCTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcacca                <  2:695039/81‑1 (MQ=255)                                                         aaCTGGCACGCGCCACTCACGCTAATATCCACCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcacc                 <  2:695038/80‑1 (MQ=255)                                                             ggCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGAACTTCCTCGTCACCACGCt            >  1:695090/1‑81 (MQ=255)                                                               cACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGt          <  1:695040/81‑1 (MQ=255)                                                                aCGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGtt         <  1:695041/81‑1 (MQ=255)                                                                  gcgcCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAg       >  1:695086/1‑81 (MQ=255)                                                                       aCTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATg  <  1:695042/81‑1 (MQ=255)                                                                               |                                                                         AACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATG  >  NC_000913/3608412‑3608562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑