Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,611,739	T→A	intergenic (‑6/‑126)	yhhS ← / → yhhT	putative transporter YhhS/putative transporter YhhT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,611,739	0	T	A	100.0%	142.5 / NA	39	intergenic (‑6/‑126)	yhhS/yhhT	putative transporter YhhS/putative transporter YhhT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/22);  total (17/22)

ATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGATCACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCAGAATATTGC  >  NC_000913/3611663‑3611815                                                                             |                                                                             aTGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAAcacc                                                                          <  1:695856/81‑1 (MQ=255)  tGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAAcacca                                                                         <  2:695857/81‑1 (MQ=255)     cTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAt                                                                      <  2:695858/81‑1 (MQ=255)      tataGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAtt                                                                     >  1:695893/1‑81 (MQ=255)        taGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAtttt                                                                   <  1:695859/81‑1 (MQ=255)        taGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAtttt                                                                   >  1:695904/1‑81 (MQ=255)         agagACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAtttt                                                                   >  2:695897/1‑80 (MQ=255)          gagaCAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACAttttt                                                                  <  2:695860/80‑1 (MQ=255)           agaCAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTa                                                                 >  2:695896/1‑80 (MQ=255)              cAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTacaac                                                             <  2:695861/81‑1 (MQ=255)                   cgcAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAgtg                                                         <  2:695862/80‑1 (MQ=255)                    gcAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGtt                                                       <  2:695863/81‑1 (MQ=255)                    gcAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGtt                                                       <  2:695864/81‑1 (MQ=255)                      aaaTTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGttt                                                      >  2:695902/1‑80 (MQ=255)                      aaaTTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATAAGGAACACCACATTTTTACAACAGTGttt                                                      >  1:695895/1‑80 (MQ=255)                           cAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCAttt                                                 <  1:695866/80‑1 (MQ=255)                           cAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCAttt                                                 <  2:695865/80‑1 (MQ=255)                               cgcAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTAcc                                            <  1:695867/81‑1 (MQ=255)                                gcAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCa                                           <  1:695868/81‑1 (MQ=255)                                gcAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCa                                           >  1:695915/1‑81 (MQ=255)                                gcAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCa                                           >  2:695901/1‑81 (MQ=255)                                    tCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTg                                       >  1:695911/1‑81 (MQ=255)                                      cGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACGCCACATTTTTACAACAGTGTTTCATTTTTACCACGTGc                                      <  2:695871/80‑1 (MQ=255)                                      cGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCt                                     <  2:695870/81‑1 (MQ=255)                                      cGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCt                                     >  1:695905/1‑81 (MQ=255)                                      cGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCt                                     <  2:695869/81‑1 (MQ=255)                                       gTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCt                                     >  1:695872/1‑80 (MQ=255)                                                  gTTCGGCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATc                         <  2:695873/81‑1 (MQ=255)                                                      ggCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATcagca                     <  1:695874/81‑1 (MQ=255)                                                       gCTACGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATcagcag                    >  2:695898/1‑81 (MQ=255)                                                           cGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTaa                 <  1:695876/80‑1 (MQ=255)                                                           cGGGTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAg                <  2:695875/81‑1 (MQ=255)                                                              gTTCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAAt             <  1:695877/81‑1 (MQ=255)                                                               ttCGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAAt             >  2:695916/1‑80 (MQ=255)                                                                 cGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAAt             >  1:695907/1‑78 (MQ=255)                                                                 cGGGCATGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCa           <  1:695878/80‑1 (MQ=255)                                                                      aTGAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCAGAatat     >  2:695912/1‑81 (MQ=255)                                                                        gAGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCAGAATATTg   >  1:695917/1‑81 (MQ=255)                                                                         aGGAACACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCAGAATATTGc  >  1:695908/1‑81 (MQ=255)                                                                             |                                                                             ATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGCATGAGGATCACCACATTTTTACAACAGTGTTTCATTTTTACCACGTGCTGACGTGAAAATCAGCAGTAAGAATCAGAATATTGC  >  NC_000913/3611663‑3611815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑