Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,168	A→T	D46E (GAT→GAA)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,168	0	A	T	97.5%	144.7 / NA	40	D46E (GAT→GAA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (18/21);  minor base G (0/1);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCC  >  NC_000913/366092‑366245                                                                             |                                                                              ccGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCg                                                                            <  2:78294/80‑1 (MQ=255)   ggCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTg                                                                         >  2:78352/1‑81 (MQ=255)      aCCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCgg                                                                      <  1:78296/81‑1 (MQ=255)      aCCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCgg                                                                      <  2:78295/81‑1 (MQ=255)         gCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGcc                                                                   >  1:78358/1‑81 (MQ=255)               ggTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCg                                                             >  1:78355/1‑81 (MQ=255)                gTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTc                                                              <  1:78297/79‑1 (MQ=255)                 tGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCt                                                           <  1:78298/81‑1 (MQ=255)                    cGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCAGGCCTCTTCGCTAtt                                                        <  2:78299/81‑1 (MQ=255)                     ggAAACCAGGCAAAGCGCCACTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTa                                                       >  2:78353/1‑81 (MQ=255)                       aaaCCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACg                                                     <  1:78300/81‑1 (MQ=255)                          ccAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGc                                                    >  2:78349/1‑79 (MQ=255)                              gCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTg                                              >  2:78359/1‑81 (MQ=255)                               cAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTgg                                             <  1:78301/81‑1 (MQ=255)                                aaaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGc                                            <  1:78302/81‑1 (MQ=255)                                aaaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGc                                            >  1:78368/1‑81 (MQ=255)                                 aaGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCg                                           <  2:78303/81‑1 (MQ=255)                                    cgccattcgccattCAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGa                                          >  2:78365/1‑79 (MQ=255)                                     gccattcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAg                                       >  1:78354/1‑81 (MQ=255)                                      ccattcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAgg                                      >  1:78367/1‑81 (MQ=255)                                         ttcgccattcAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAggggg                                   <  1:78304/81‑1 (MQ=255)                                            gccattcAGGCTGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAtg                                >  2:78369/1‑81 (MQ=255)                                                       tGCGCAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGc                     >  1:78361/1‑81 (MQ=255)                                                         cgcAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGc                     <  2:78305/79‑1 (MQ=255)                                                          gcAACTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGAt                  <  1:78306/81‑1 (MQ=255)                                                           cAACTGTTGGGTAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGAtt                 <  1:78307/81‑1 (MQ=255)                                                            aaCTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTa                <  1:78308/81‑1 (MQ=255)                                                              cTGTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAg              <  1:78309/81‑1 (MQ=255)                                                                gTTGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGtt            >  1:78357/1‑81 (MQ=255)                                                                  tGGGAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTgg          >  1:78360/1‑81 (MQ=255)                                                                   gggAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTggg         >  2:78356/1‑81 (MQ=255)                                                                    ggAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGt        <  2:78310/81‑1 (MQ=255)                                                                     gAAGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGt        >  2:78372/1‑80 (MQ=255)                                                                       aGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAAc     <  2:78311/81‑1 (MQ=255)                                                                       aGGGCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAAc     >  1:78366/1‑81 (MQ=255)                                                                        gggCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACg    >  2:78370/1‑81 (MQ=255)                                                                         ggCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGc   <  1:78312/81‑1 (MQ=255)                                                                         ggCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGc   <  1:78313/81‑1 (MQ=255)                                                                          gCGTTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGcc  <  2:78314/81‑1 (MQ=255)                                                                          gCGGTCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGcc  <  2:78315/81‑1 (MQ=255)                                                                             |                                                                              CCGGCACCGCTTCTGGTGCCGGAAACCAGGCAAAGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCC  >  NC_000913/366092‑366245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑