Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,704,055	G→A	A236V (GCT→GTT)	dppC ←	dipeptide ABC transporter membrane subunit DppC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,704,055	0	G	A	100.0%	133.4 / NA	37	A236V (GCT→GTT)	dppC	dipeptide ABC transporter membrane subunit DppC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

GAACTGCAACACGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAGCAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCGGGAAGATG  >  NC_000913/3703979‑3704132                                                                             |                                                                              gAACTGCAACACGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGaacagc                                                                           >  1:717212/1‑81 (MQ=255)  aaCTGCAACACGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGcc                                                                          <  1:717168/81‑1 (MQ=255)       cAACACGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATc                                                                     <  2:717169/81‑1 (MQ=255)         acacGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCTATGCCGAGGAAACCAAGAACAGCCATATc                                                                     >  2:717220/1‑79 (MQ=255)            cGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGaa                                                                <  1:717170/81‑1 (MQ=255)             gTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAAt                                                               >  2:717219/1‑81 (MQ=255)                ggAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGc                                                            >  1:717221/1‑81 (MQ=255)                   gagCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGtt                                                         <  2:717171/81‑1 (MQ=255)                    agCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGt                                                          >  1:717226/1‑79 (MQ=255)                       aTGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTagag                                                     <  2:717173/81‑1 (MQ=255)                       aTGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTagag                                                     <  1:717172/81‑1 (MQ=255)                        tGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAgaga                                                    <  1:717174/81‑1 (MQ=255)                          gTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGaa                                                   <  2:717175/80‑1 (MQ=255)                            gCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAAcc                                                >  2:717256/1‑81 (MQ=255)                             ccacACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAAcc                                                <  2:717176/77‑1 (MQ=255)                                cACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGc                                            <  2:717177/81‑1 (MQ=255)                                 aCTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGc                                            >  1:717239/1‑80 (MQ=255)                                     aGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGAc                                         <  1:717178/79‑1 (MQ=255)                                     aGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGc                                       <  1:717179/81‑1 (MQ=255)                                         gTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTg                                    >  2:717234/1‑80 (MQ=255)                                         gTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTg                                    >  1:717242/1‑80 (MQ=255)                                         gTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTg                                    >  2:717243/1‑80 (MQ=255)                                            ggcggcTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAAc                                 >  2:717240/1‑80 (MQ=255)                                            ggcggcTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAAc                                 >  2:717229/1‑80 (MQ=255)                                               ggcTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAAt                              >  1:717236/1‑80 (MQ=255)                                               ggcTGTGCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATc                             <  2:717180/81‑1 (MQ=255)                                                    tgCCCCCATGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAgc                          <  2:717181/79‑1 (MQ=255)                                                            tGCCGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGc                <  1:717182/81‑1 (MQ=255)                                                               cGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGt             >  2:717247/1‑81 (MQ=255)                                                               cGAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGt             >  1:717260/1‑81 (MQ=255)                                                                gAGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGtt            >  2:717227/1‑81 (MQ=255)                                                                 aGGAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTc           <  1:717183/81‑1 (MQ=255)                                                                  ggAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCg          >  2:717237/1‑81 (MQ=255)                                                                   gAAACCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCgg         <  2:717184/81‑1 (MQ=255)                                                                    aaaCCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCgg         >  2:717233/1‑80 (MQ=255)                                                                      aCCAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCGGGaa      <  1:717185/81‑1 (MQ=255)                                                                        cAAGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCggg        >  2:717235/1‑77 (MQ=255)                                                                         aaGAACAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCGGGAAGAt   >  1:717230/1‑81 (MQ=255)                                                                          agaaCAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCGGGAAGATg  >  2:717282/1‑81 (MQ=255)                                                                             |                                                                              GAACTGCAACACGTCGGAGAGCATGGTGCCCCACTCAGGCGTTGGCGGCTGTGCCCCCATGCCGAGGAAACCAAGAGCAGCCATATCGAGAATGGCGTTAGAGAAACCGAGCGACGCCTGAACAATCAGCGGCGCAAGGCAGTTCGGGAAGATG  >  NC_000913/3703979‑3704132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑