Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,734,789	T→A	L357Q (CTG→CAG)	xylH →	xylose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,734,789	0	T	A	100.0%	112.5 / NA	31	L357Q (CTG→CAG)	xylH	xylose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/15);  total (16/15)

TTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACTGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTG  >  NC_000913/3734714‑3734862                                                                            |                                                                          ttGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGAt                                                                       >  1:724147/1‑80 (MQ=255) ttGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATa                                                                      <  1:724091/81‑1 (MQ=255)   ggcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAAc                                                                    >  1:724137/1‑81 (MQ=255)    gcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAAc                                                                    <  1:724092/80‑1 (MQ=255)    gcggcACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACg                                                                   <  1:724093/81‑1 (MQ=255)         aCCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATg                                                              <  1:724094/81‑1 (MQ=255)            aGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGt                                                           <  1:724095/81‑1 (MQ=255)             gCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTa                                                          >  2:724131/1‑81 (MQ=255)              ccTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTat                                                         >  2:724139/1‑81 (MQ=255)                  gctggcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTatgat                                                      <  2:724096/80‑1 (MQ=255)                      gcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg                                                 >  2:724151/1‑81 (MQ=255)                      gcGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg                                                 >  1:724143/1‑81 (MQ=255)                       cGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATgatggatg                                                 >  1:724149/1‑80 (MQ=255)                       cGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGt                                                <  1:724097/81‑1 (MQ=255)                         gtgtGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTAc                                              <  2:724098/81‑1 (MQ=255)                              ggAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGAcc                                         >  2:724138/1‑81 (MQ=255)                                 aGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTc                                      >  1:724140/1‑81 (MQ=255)                                  gCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCt                                     <  2:724100/81‑1 (MQ=255)                                  gCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCt                                     <  2:724099/81‑1 (MQ=255)                                    gTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTgg                                   >  1:724142/1‑81 (MQ=255)                                     ttGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTgg                                   >  1:724146/1‑80 (MQ=255)                                     ttGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGc                                  <  1:724101/81‑1 (MQ=255)                                       gCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCa                                 <  1:724102/80‑1 (MQ=255)                                           gAGCAGTAATGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGtata                            >  1:724141/1‑81 (MQ=255)                                                  aaTGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTa                      >  1:724150/1‑80 (MQ=255)                                                   aTGGGGGCATTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTaa                     <  1:724103/80‑1 (MQ=255)                                                           aTTTATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTCCTGGCAGTATATCGTTAAAGGTGCGAt            >  1:724148/1‑81 (MQ=255)                                                            tttATCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGAtt           >  1:724152/1‑81 (MQ=255)                                                               aTCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTg        >  2:724161/1‑81 (MQ=255)                                                                tCATGGCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGt       <  2:724104/81‑1 (MQ=255)                                                                     gCTTCACAGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTg  <  1:724105/81‑1 (MQ=255)                                                                            |                                                                          TTGGCGGCACCAGCCTGGCTGGCGGTGTGGGAAGCGTTGCCGGAGCAGTAATGGGGGCATTTATCATGGCTTCACTGGATAACGGCATGAGTATGATGGATGTACCGACCTTCTGGCAGTATATCGTTAAAGGTGCGATTCTGTTGCTG  >  NC_000913/3734714‑3734862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑