Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,740,658	C→A	I318I (ATC→ATA)	avtA →	valine‑‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,658	0	C	A	100.0%	92.5 / NA	26	I318I (ATC→ATA)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/11);  total (15/11)

ATTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT  >  NC_000913/3740581‑3740734                                                                              |                                                                             aTTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGc                                                                            >  1:725474/1‑80 (MQ=255)    aaGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcat                                                                         >  1:725490/1‑80 (MQ=255)     aGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcatca                                                                       <  1:725446/81‑1 (MQ=255)          aaCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgc                                                                  >  1:725487/1‑81 (MQ=255)           aCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgc                                                                  <  1:725447/80‑1 (MQ=255)            cGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgccg                                                                <  1:725448/81‑1 (MQ=255)            cGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgccg                                                                >  1:725486/1‑81 (MQ=255)             gATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgccgc                                                               >  1:725481/1‑81 (MQ=255)               tCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTa                                                             >  2:725472/1‑81 (MQ=255)                ctgctgCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGCTCAGGAAACTATAGCCATCATTCGCCGCTAt                                                            >  2:725475/1‑81 (MQ=255)                    tgcGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTAc                                                        >  1:725485/1‑81 (MQ=255)                                        atcaAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGAtt                                    >  2:725491/1‑81 (MQ=255)                                         tcaAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTc                                   <  1:725449/81‑1 (MQ=255)                                              ccGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATaaa                              >  1:725488/1‑81 (MQ=255)                                                gTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAAcc                            >  2:725483/1‑81 (MQ=255)                                                     actacCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAg                       >  1:725479/1‑81 (MQ=255)                                                      ctacCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAg                       >  1:725482/1‑80 (MQ=255)                                                          cAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCAGAAGGAGcc                  <  2:725450/81‑1 (MQ=255)                                                           aGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCa                 <  2:725451/81‑1 (MQ=255)                                                            gCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAt                >  2:725484/1‑81 (MQ=255)                                                                   cAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCct          <  2:725452/80‑1 (MQ=255)                                                                    aGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCctct        <  2:725453/81‑1 (MQ=255)                                                                        aaCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGc     >  2:725492/1‑80 (MQ=255)                                                                          cTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAt  <  2:725456/81‑1 (MQ=255)                                                                          cTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAt  <  1:725455/81‑1 (MQ=255)                                                                          cTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAt  <  1:725454/81‑1 (MQ=255)                                                                              |                                                                             ATTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT  >  NC_000913/3740581‑3740734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑