Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,820,029	A→G	V381A (GTG→GCG)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,029	0	A	G	100.0%	114.2 / NA	32	V381A (GTG→GCG)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

CAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCC  >  NC_000913/3819958‑3820106                                                                        |                                                                              cAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCAcatcat                                                                      <  2:742967/81‑1 (MQ=255)        gCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGc                                                                <  2:742968/80‑1 (MQ=255)           ggTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAg                                                             >  2:743025/1‑80 (MQ=255)            gTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAgg                                                            >  2:743013/1‑80 (MQ=255)               aaCGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATa                                                        >  1:743011/1‑81 (MQ=255)                    gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTg                                                   <  1:742969/81‑1 (MQ=255)                     ttAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGa                                                  >  1:743014/1‑81 (MQ=255)                         aGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCgg                                              <  1:742970/81‑1 (MQ=255)                             gTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAg                                          <  1:742971/81‑1 (MQ=255)                                 tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAGTACCCTGACCGGCAAGGCt                                       <  1:742972/80‑1 (MQ=255)                                 tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCt                                       <  2:742973/80‑1 (MQ=255)                                     gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa                                     <  1:742974/78‑1 (MQ=255)                                     gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc                                    <  2:742975/79‑1 (MQ=255)                                        gtgtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGaa                               >  1:743032/1‑81 (MQ=255)                                             ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTg                           <  2:742976/80‑1 (MQ=255)                                             ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGa                          >  1:743039/1‑81 (MQ=255)                                             ggTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGa                          <  2:742977/81‑1 (MQ=255)                                              gTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGAt                         >  2:743016/1‑81 (MQ=255)                                               ttttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATc                        <  2:742978/81‑1 (MQ=255)                                                 ttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCa                       <  1:742980/80‑1 (MQ=255)                                                 ttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCa                       <  2:742979/80‑1 (MQ=255)                                                  tGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCAc                      <  2:742981/80‑1 (MQ=255)                                                    tACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAg                   <  1:762548/81‑1 (MQ=255)                                                       gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg                <  2:742982/81‑1 (MQ=255)                                                       gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg                >  2:743021/1‑81 (MQ=255)                                                        ttCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcgc               <  1:742983/81‑1 (MQ=255)                                                          cTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCaa             >  2:743036/1‑81 (MQ=255)                                                             cACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAat            <  2:742984/79‑1 (MQ=255)                                                               ccGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc        >  2:743048/1‑81 (MQ=255)                                                                cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc        >  2:743018/1‑80 (MQ=255)                                                                cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc        >  2:743037/1‑80 (MQ=255)                                                                 gcgcCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCa      >  1:743027/1‑81 (MQ=255)                                                                   gccacgccacATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACt    >  2:743035/1‑81 (MQ=255)                                                                     cacgccacaTCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTcc  <  2:742986/81‑1 (MQ=255)                                                                     cacgccacaTCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTcc  <  1:742985/81‑1 (MQ=255)                                                                        |                                                                              CAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACTCC  >  NC_000913/3819958‑3820106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑