Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,019	C→T	V173V (GTG→GTA)	yicI ←	alpha‑D‑xyloside xylohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,019	0	C	T	100.0%	165.6 / NA	45	V173V (GTG→GTA)	yicI	alpha‑D‑xyloside xylohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/26);  total (19/26)

TATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAA  >  NC_000913/3833949‑3834094                                                                       |                                                                            tatTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAg                                                                   >  2:746621/1‑81 (MQ=255)   tttttATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGt                                                                  <  2:746568/80‑1 (MQ=255)     tttATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTaa                                                                <  1:746569/80‑1 (MQ=255)         taCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgcgc                                                           >  1:746619/1‑81 (MQ=255)         taCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgcg                                                            <  2:746570/80‑1 (MQ=255)          aCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGct                                                          >  1:746628/1‑81 (MQ=255)              cTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTcc                                                      <  2:746571/81‑1 (MQ=255)                 ttCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAg                                                   <  1:746572/81‑1 (MQ=255)                   cAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc                                                 >  2:746639/1‑81 (MQ=255)                    aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAcc                                                <  2:746574/81‑1 (MQ=255)                    aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAcc                                                <  1:746573/81‑1 (MQ=255)                      tACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCg                                               >  2:746622/1‑80 (MQ=255)                       aCTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGt                                              >  2:746617/1‑80 (MQ=255)                        cTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTa                                             <  2:746575/80‑1 (MQ=255)                           gtgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAAc                                          <  1:746576/80‑1 (MQ=255)                           gtgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACt                                         >  2:746635/1‑81 (MQ=255)                            tgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTg                                        <  1:746577/81‑1 (MQ=255)                               cgccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGttt                                     <  1:746578/81‑1 (MQ=255)                                gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                    <  1:746580/81‑1 (MQ=255)                                gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                    <  2:746579/81‑1 (MQ=255)                                gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                    >  1:746637/1‑81 (MQ=255)                                gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                    >  1:746633/1‑81 (MQ=255)                                gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                    <  1:746581/81‑1 (MQ=255)                                 ccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcg                                   <  2:746583/81‑1 (MQ=255)                                 ccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcg                                   <  1:746582/81‑1 (MQ=255)                                  cgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgc                                  >  2:746632/1‑81 (MQ=255)                                   gTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgcc                                 <  1:746584/81‑1 (MQ=255)                                   gTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgc                                  >  1:746615/1‑80 (MQ=255)                                       cGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacg                             <  2:746585/81‑1 (MQ=255)                                        ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgc                            <  2:746586/81‑1 (MQ=255)                                        ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgc                            >  1:746626/1‑81 (MQ=255)                                        ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgc                            >  2:746625/1‑81 (MQ=255)                                         gTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcc                           <  1:746587/81‑1 (MQ=255)                                          ttCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcca                          <  1:746588/81‑1 (MQ=255)                                            ccAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcc                           >  2:746623/1‑78 (MQ=255)                                             cAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcc                           >  2:746630/1‑77 (MQ=255)                                              aGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcc                           >  2:746650/1‑76 (MQ=255)                                               ggTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc                     <  2:746589/81‑1 (MQ=255)                                                  ctctACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAg                  >  1:746634/1‑81 (MQ=255)                                                     tACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAg                  <  1:746590/78‑1 (MQ=255)                                                       ccGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCg               <  2:746591/79‑1 (MQ=255)                                                           cTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCaaa         >  2:746643/1‑81 (MQ=255)                                                              gCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACat      <  1:746592/81‑1 (MQ=255)                                                                 aTTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACatat    >  1:746644/1‑80 (MQ=255)                                                                  ttGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATaa  <  2:746593/81‑1 (MQ=255)                                                                       |                                                                            TATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAA  >  NC_000913/3833949‑3834094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑