Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,864,243	C→T	I115I (ATC→ATT)	yidP →	putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,864,243	0	C	T	100.0%	111.0 / NA	31	I115I (ATC→ATT)	yidP	putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/19);  total (12/19)

ACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCTATATGCC  >  NC_000913/3864166‑3864320                                                                              |                                                                              aCGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAAc                                                                            >  2:753622/1‑81 (MQ=255) aCGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAAc                                                                            <  2:753572/81‑1 (MQ=255)        ccagccagGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAg                                                                         >  1:753620/1‑77 (MQ=255)         cagccagGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGAt                                                                    <  1:753573/81‑1 (MQ=255)          agccagGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATc                                                                   >  2:753616/1‑81 (MQ=255)          agccagGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATc                                                                   <  2:753574/81‑1 (MQ=255)           gccagGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATc                                                                   >  2:753618/1‑80 (MQ=255)              agGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACt                                                               <  2:753575/81‑1 (MQ=255)               ggTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACtt                                                              >  2:753614/1‑81 (MQ=255)                     gATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCg                                                        >  1:753621/1‑81 (MQ=255)                      aTTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCg                                                        <  1:753576/80‑1 (MQ=255)                      aTTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGt                                                       <  1:753577/81‑1 (MQ=255)                      aTTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGt                                                       <  1:753578/81‑1 (MQ=255)                       tttttGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTc                                                      <  2:753579/81‑1 (MQ=255)                             aaaTCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGcgtt                                                 <  2:753580/80‑1 (MQ=255)                               aTCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGcgttcgt                                              <  2:753581/81‑1 (MQ=255)                                tCATGCCTGCGCCTCCGGACATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGcgttcgtt                                             <  1:753582/81‑1 (MQ=255)                                    gCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTtgt                                         >  1:753615/1‑81 (MQ=255)                                     ccTGCGCCTCCGGTCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTtgt                                         <  2:753583/80‑1 (MQ=255)                                         cgcCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGa                                      <  2:753584/79‑1 (MQ=255)                                            cTCCGGCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAgtg                                  >  2:753628/1‑78 (MQ=255)                                                ggCCATTGCCAGCCAGTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAAcc                             >  2:753625/1‑81 (MQ=255)                                                            ccagTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTgg                  >  1:753627/1‑80 (MQ=255)                                                            ccagTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGa                 <  2:753585/81‑1 (MQ=255)                                                             cagTTACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTgg                  >  2:753617/1‑79 (MQ=255)                                                                ttACGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGAc             <  2:753586/81‑1 (MQ=255)                                                                   cGGATTCAAATTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGTAAACCGCTGATGCTGGAAGACAg           <  1:753587/80‑1 (MQ=255)                                                                       ttCAAATTAACGAGCAGATCTACTTCTCCCGACGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCtata      <  2:753588/81‑1 (MQ=255)                                                                          aaaTTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCTATATg    <  1:753589/80‑1 (MQ=255)                                                                           aaTTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCTATATGcc  <  1:753590/81‑1 (MQ=255)                                                                           aaTTAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCTATATGcc  >  2:753629/1‑81 (MQ=255)                                                                              |                                                                              ACGGTCACCAGCCAGGTGCTGATTTTTGAAATCATGCCTGCGCCTCCGGCCATTGCCAGCCAGTTACGGATTCAAATCAACGAGCAGATCTACTTCTCCCGTCGCGTTCGTTTTGTGGAAGGGAAACCGCTGATGCTGGAAGACAGCTATATGCC  >  NC_000913/3864166‑3864320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑