Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,921,309	A→T	I248N (ATC→AAC)	atpB ←	ATP synthase Fo complex subunit a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,921,309	0	A	T	100.0%	174.3 / NA	47	I248N (ATC→AAC)	atpB	ATP synthase Fo complex subunit a
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (32/15);  total (32/15)

AATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAAT  >  NC_000913/3921234‑3921385                                                                            |                                                                             aaTTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAg                                                                          >  1:767277/1‑80 (MQ=255)  aTTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGa                                                                        >  2:767291/1‑81 (MQ=255)    tAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGAtg                                                                      >  1:767284/1‑81 (MQ=255)         ttcttcAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGa                                                                  >  1:767293/1‑80 (MQ=255)         ttcttcAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGa                                                                  <  1:767221/80‑1 (MQ=255)             tcAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATg                                                             >  1:767305/1‑81 (MQ=255)             tcAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATg                                                             >  1:767274/1‑81 (MQ=255)                gACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGcc                                                          >  2:767272/1‑81 (MQ=255)                  cGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCa                                                        <  2:767222/81‑1 (MQ=255)                   gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCAc                                                       >  1:767299/1‑81 (MQ=255)                   gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCAc                                                       >  2:767269/1‑81 (MQ=255)                    ccATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACg                                                      >  1:767262/1‑81 (MQ=255)                      aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAACATGGCCCACgg                                                     >  2:767281/1‑80 (MQ=255)                      aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACgg                                                     >  2:767306/1‑80 (MQ=255)                      aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGc                                                    <  1:767224/81‑1 (MQ=255)                      aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGc                                                    <  1:767223/81‑1 (MQ=255)                       tCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGca                                                   >  1:767268/1‑81 (MQ=255)                        cGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGca                                                   >  2:767276/1‑80 (MQ=255)                        cGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGca                                                   >  2:767303/1‑80 (MQ=255)                           cAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACAtt                                               <  2:767225/81‑1 (MQ=255)                            aGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTc                                              <  2:767226/81‑1 (MQ=255)                             gATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACAtt                                               >  2:767273/1‑79 (MQ=255)                               tAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAgg                                           <  2:767227/81‑1 (MQ=255)                               tAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAg                                            >  1:767275/1‑80 (MQ=255)                                 gACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGa                                          <  2:767228/80‑1 (MQ=255)                                     aTCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCAc                                     <  2:767229/81‑1 (MQ=255)                                     aTCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCAc                                     >  1:767298/1‑81 (MQ=255)                                        gTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACtgt                                  >  2:767278/1‑81 (MQ=255)                                        gTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACtgt                                  >  1:767279/1‑81 (MQ=255)                                        gTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACtg                                   <  2:767230/80‑1 (MQ=255)                                          cAGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGa                                >  1:767295/1‑81 (MQ=255)                                           aGAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAACGGCCCACGGCACATTCAGGATCCACTGTGac                               >  2:767290/1‑81 (MQ=255)                                            gAACCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGacc                              <  2:767231/81‑1 (MQ=255)                                              aCCATGAAGATGAAGGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGaccac                            >  1:767257/1‑81 (MQ=255)                                                 atgaagatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACcaccac                         >  1:767263/1‑81 (MQ=255)                                                 atgaagatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACcaccac                         <  1:767232/81‑1 (MQ=255)                                                    aagatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACgg                       >  2:767297/1‑80 (MQ=255)                                                    aagatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACgg                       >  2:767301/1‑80 (MQ=255)                                                     agatgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGc                      >  1:767292/1‑80 (MQ=255)                                                       atgaagGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAAc                   <  2:767233/81‑1 (MQ=255)                                                           agGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGAc               >  1:767283/1‑81 (MQ=255)                                                           agGCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGAc               <  2:767234/81‑1 (MQ=255)                                                            ggCTTGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGAcc              <  2:767235/81‑1 (MQ=255)                                                                tGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGc           >  2:767300/1‑80 (MQ=255)                                                                tGCAGCGTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGc           >  1:767286/1‑80 (MQ=255)                                                                      gTAATGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAg     >  1:767282/1‑80 (MQ=255)                                                                         aTGTTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAAt  >  2:767313/1‑80 (MQ=255)                                                                            |                                                                             AATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAAT  >  NC_000913/3921234‑3921385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑